Abstract/Details

Raman and infrared study of electrospun PLLA/PCL nanofiber blends for use in tissue engineering


2012 2012

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Abstract (summary)

Recently, the biomedical engineering field has developed at a very fast pace as improved techniques and materials become available to promote its growth. Consequently, the research in polymeric biomaterials has been highly stimulated by this trend. The goal of the current research is to demonstrate the usefulness of the Raman scattering, Raman mapping, and infrared absorption spectroscopies to tissue engineering, by spectroscopically characterizing blends of PLLA and PCL polymers, which were prepared by electrospinning with and without cell addition. The proposed use of these blends is as primary biomaterials in biodegradable scaffolds used in tissue engineering. Both Raman and infrared absorption spectra showed a direct relation between the relative intensities of the characteristic molecular vibrations of the individual polymers with their concentrations in each blend. The confocal Raman mapping of the samples that were prepared by co-electrospinning allowed direct visualization of different polymeric fibers. These images not only reveal the microstructural characteristics of each polymer, but they are also in good agreement with the Raman scattering results. Furthermore, by performing Raman mapping inside a single fiber, the homogeneity of the polymeric mixture can be visualized. These results demonstrate the existence of sub-domains of non-interacting polymers. The broadening of the cell characteristic peak at 1661 cm -1 observed in the Raman spectra of the blends seeded with C2C12 myoblasts, could be an indication of cell attachment onto the scaffolds.

Indexing (details)


Subject
Polymer chemistry;
Biomedical engineering;
Biophysics;
Materials science
Classification
0495: Polymer chemistry
0541: Biomedical engineering
0786: Biophysics
0794: Materials science
Identifier / keyword
Pure sciences; Biological sciences; Applied sciences; Electrospinning; Myoblasts; Pcl; Plla; Polymeric biomaterials; Raman; Scaffolds
Title
Raman and infrared study of electrospun PLLA/PCL nanofiber blends for use in tissue engineering
Author
Enriquez Carrejo, Jose Luis
Number of pages
90
Publication year
2012
Degree date
2012
School code
0459
Source
DAI-B 74/01(E), Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9781267593054
Advisor
Manciu, Felicia S.
Committee member
Chianelli, Russell R.; Durrer, William G.; Murr, Lawrence E.; Xu, Tao
University/institution
The University of Texas at El Paso
Department
Matl. Sci. And Engin.
University location
United States -- Texas
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3525751
ProQuest document ID
1080792225
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/1080792225
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