Correction: Bions: A Family of Biomimetic Mineralo-Organic Complexes Derived from Biological Fluids

Abstract

Bions sub-cultures were incubated in cell culture conditions for 2 weeks and the amount of bions was monitored by light absorbance. Bions were retrieved by centrifugation, followed by washing steps and preparation for EDX analysis.

There is an error in the Sr-Bions graph of Figure 10C. Please see the corrected Figure 10 here.

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Figure 10:. Increase in size and number, and sub-culture of bions.

(A) Bions increase in size during incubation. Bions were prepared by adding 1 mM CaCl2, 10 mM MgCl2, 1 mM MnCl2, 5 mM SrCl2 or 3 mM BaCl2 in DMEM containing 5% FBS, followed by addition of Na2HPO4 at the same concentration to induce precipitation. Particle size was determined by DLS during a 2-week incubation period. (B) Bions increase in number in culture. Bions were prepared and incubated as described in (A) and particle number was determined by DLS. (C) Bions are sub-culturable in fresh medium. Bions were subcultured by diluting a solution containing abundant, pre-formed bions (A650 of 0.7) in fresh DMEM containing 10% FBS at a ratio of 1?10 using a final volume of 15 ml. Bions sub-cultures were incubated in cell culture conditions for 2 weeks and the amount of bions was monitored by light absorbance. (D) EDX analysis of bions after sub-culture and incubation for 2 weeks. Bions were retrieved by centrifugation, followed by washing steps and preparation for EDX analysis. See the text for more details.

https://doi.org/10.1371/journal.pone.0075501.g010

References

1. Wu C-Y, Young L, Young D, Martel J, Young JD (2013) Bions: A Family of Biomimetic Mineralo-Organic Complexes Derived from Biological Fluids. PLoS ONE 8(9): e75501