Content area
Full Text
Appl Biochem Biotechnol (2015) 175:28952906
DOI 10.1007/s12010-014-1461-6
Ramachandran Srinivasan &
Velayutham Arumuga Kumar & Dileep Kumar & Nachimuthu Ramesh & Subramanian Babu & Kodiveri Muthukalianan Gothandam
Received: 26 May 2014 /Accepted: 21 December 2014 /
Published online: 10 January 2015# Springer Science+Business Media New York 2015
Abstract This study aimed to explore the effect of sodium bicarbonate (0200 mM) on the production of -carotene and lipid content in Dunaliella salina and Dunaliella bardawil. Total carotenoid and chlorophyll content were determined at regular intervals by a UVVIS spectrophotometer. The -carotene and lipid contents were analyzed using high-performance liquid chromatography (HPLC) and gas chromatography coupled with mass spectrometry (GC-MS). The HPLC results revealed a twofold increase of -carotene in D. salina andD. bardawil cultures grown with sodium bicarbonate. Moreover, total fatty acid profiles from GC-MS indicated a maximum relative percentage of saturated fatty acids (tetradecanoic acid, 10,13-diethyl, methyl ester and methyl 16-methyl-heptadecanoate) compared to polyunsatu-rated fatty acids in both algae. Our results indicate that the optimum concentration of bicarbonate (100 to 150 mM) was required to stimulate a positive effect on -carotene production as well as the lipid profile in Dunaliella sp.
Keywords -Carotene . Chlorophyll . Dunaliella salina . Dunaliella bardawil . Bicarbonate . Saturated fatty acids
Introduction
Nutritional modifications such as limitation, depletion, and supplementation in culture media, plays a major role in enhancing the biochemical constituents of marine microalgae. Microalgae have the ability to adapt their cellular and physiological patterns with respect to nutrient availability by altering the metabolic pathways resulting in the production of several metabolites such as lipids, carbohydrates, and proteins. The ability of algae to survive under extreme conditions causes unusual changes in the level of intracellular components and lipid content [34]. Since algae have the ability to survive under extreme conditions and also produce acyl and nonacyl lipids (carotenoids), further modification in the media components might result in
R. Srinivasan : V. A. Kumar : D. Kumar : N. Ramesh : S. Babu : K. M. Gothandam (*) School of Bio-Sciences and Technology, VIT University, Vellore 632 014 Tamil Nadu, India e-mail: [email protected]
Effect of Dissolved Inorganic Carbon on -Carotene and Fatty Acid Production in Dunaliella sp
2896 Appl Biochem Biotechnol (2015) 175:28952906
the improvement of the quality of nutritionally...