Abstract/Details

THE MOLECULAR ANALYSIS OF THE ACE GENE IN DROSOPHILA


1986 1986

Other formats: Order a copy

Abstract (summary)

The rapid hydrolysis of the neurotransmitter, acetylcholine, is mediated by the enzyme acetylcholinesterase (AChE). This function is believed to be critical for the normal function of cholinergic neurotransmission. In Drosophila, AChE expression in nervous tissue is necessary for viability, behavior, and proper development of the nervous system. Genetic mosaic flies which are homozygous Ace('-) for portions of their nervous system can display abnormal behavior as well as morphological defects in their nervous tissue. AChE inactivation at specific times in the life cycle often result in death or behavioral abnormalities. The multimeric AChE enzyme is polymorphic in situ, having both soluble and hydrophobic forms. Evidence for 4-5 different catalytic subunits exists, some of these forms are specifically inactivated by Ace lethals in an allele specific manner.

The likely structural gene for AChE, Ace, is a large gene with a complex transcriptional pattern. By deletion mapping, Ace lies between +17 - +59 and is at least 16 kb in size. Recombinational analysis of cytologically normal Ace('-) alleles have identified sequences essential for Ace('+) expression. These regions have homology to four transcripts, three of which have a size and developmental profile consistent with the properties of AChE. The transcripts appear to be differentially spliced, with the putative exons possibly extending over as much as 20 kb. The organization of transcriptional activity in the Ace region and its relationship to AChE expression and structure is considered. Exceptional Ace('-) mutants recovered from the recombinational mapping experiments are also discussed.

Indexing (details)


Subject
Biology
Classification
0306: Biology
Identifier / keyword
Biological sciences
Title
THE MOLECULAR ANALYSIS OF THE ACE GENE IN DROSOPHILA
Author
NAGOSHI, RODNEY NAGATOSHI
Number of pages
260
Publication year
1986
Degree date
1986
School code
0084
Source
DAI-B 47/03, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
University/institution
Harvard University
University location
United States -- Massachusetts
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
8609750
ProQuest document ID
303469674
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/303469674/abstract
Access the complete full text

You can get the full text of this document if it is part of your institution's ProQuest subscription.

Try one of the following:

  • Connect to ProQuest through your library network and search for the document from there.
  • Request the document from your library.
  • Go to the ProQuest login page and enter a ProQuest or My Research username / password.