Abstract/Details

Molecular genetic analysis of N-cadherin structure and function


1996 1996

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Abstract (summary)

The Ca$\sp{2+}$-dependent cell adhesion molecule, N-cadherin, plays an important role in the development of the embryo. N-cadherin is the major cell adhesion molecule in specific tissues such as the neural retina and brain, although it is widely expressed throughout many developing tissues. In a step to understand the importance of this molecule, the mechanism by which N-cadherin is regulated has been studied.

In order to further characterize the structure and function of NCAD90, we have utilized Pichia pastoris to express a recombinant form of the NCAD90 protein in a readily accessible form.

To investigate the role or N-cadherin during retinal development, a photoreceptor specific promoter for bovine arrestin was inserted upstream of all N-cadherin with a deletion in the cytoplasmic (C-terminal) domain. An SV40 polyA sequence was inserted downstream of this truncated gene construct. A control construct was created with the promoter upstream of a $\beta$-galactosidase reporter gene. (Abstract shortened by UMI.)

Indexing (details)


Subject
Molecular biology
Classification
0307: Molecular biology
Identifier / keyword
Biological sciences; Pichia pastoris
Title
Molecular genetic analysis of N-cadherin structure and function
Author
Rock, Matthew James
Number of pages
90
Publication year
1996
Degree date
1996
School code
0272
Source
MAI 35/01M, Masters Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9780591102741, 0591102749
Advisor
Grunwald, G. B.
University/institution
Thomas Jefferson University
University location
United States -- Pennsylvania
Degree
M.S.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
1381334
ProQuest document ID
304315245
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/304315245
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