Abstract/Details

IR-induced apoptosis in normal and A-T LCLs


2008 2008

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Abstract (summary)

Ataxia-telangiectasia (A-T) is a disorder where patients are unable to use ataxia-telangiectasia, mutated (ATM) to recognize and initiate DNA repair. We examined the effects of ionizing radiation (IR) on normal and A-T lymphoblastoid cell lines (LCLs) on apoptosis. LCLs were irradiated to damage DNA, incubated and collected to chronicle the effects of IR on apoptosis, with or without Protein Phosphatase 2A (PP2A) inhibitor okadaic acid (OA). Through the TUNEL assay, normal LCLs exhibited the highest apoptotic activity on day 1 following IR treatment, whereas A-T LCLs demonstrated no significant apoptotic response. Our findings suggest that ATM was responsible for the rapid response to double-stranded breaks (DSB). Preliminary results suggested that normal LCLs treated with OA, with or without IR, exhibited significantly decreased apoptotic activity when compared to normal LCLs treated with IR only. These results proposed that PP2A activity was necessary for the proper function of the IR-induced apoptotic pathway.

Indexing (details)


Subject
Cellular biology
Classification
0379: Cellular biology
Identifier / keyword
Biological sciences
Title
IR-induced apoptosis in normal and A-T LCLs
Author
Rodriguez, Armando D.
Number of pages
39
Publication year
2008
Degree date
2008
School code
0582
Source
MAI 47/04M, Masters Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9780549977124
University/institution
California State University, Dominguez Hills
University location
United States -- California
Degree
M.S.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
1461590
ProQuest document ID
304364209
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/304364209
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