Abstract/Details

Cloning of a sialyltransferase gene to analyze surface sialic acid effects on metastasis


2008 2008

Other formats: Order a copy

Abstract (summary)

ST3Gal1 is a sialyltransferase, an enzyme that adds the sugar sialic acid to chains of other carbohydrates. It has an affinity for the disaccharide, Thomsen-Friedenreich Antigen (TF-Ag), a tumor-associated carbohydrate prevalent on many types of tumor cells, so that it preferentially adds sialic acid to TF-Ag. TF-Ag is involved in tumor cell metastasis. Previous research in this laboratory found that there is differential up-regulation of ST3Gal1 expression in a nonmetastatic breast tumor cell line in comparison to a related metastatic breast tumor cell line. The aims of this research are: (1) clone the involved ST3Gal1 cDNA, (2) add this cloned sequence to the metastatic cell line, and (3) determine in vitro if it lowers cell adhesion and thus the metastatic potential.

TF-Ag is in a cryptic form in normal cells due to masking by sialic acid residues, where as in cancer cells it is not masked. TF-Ag binds to molecules on the inside of blood vessels, helping the tumor cells migrate to other organs, thus playing a role in metastasis. Metastatic potential can be measured in an animal model, but this research includes attempts to optimize an in vitro model in which cells from blood vessels are grown in culture and the binding of tumor cells to these cells is measured.

The prime focus of this research is aimed at creating a potential therapy for breast cancer and its metastasis.

Indexing (details)


Subject
Molecular biology;
Genetics;
Immunology
Classification
0307: Molecular biology
0369: Genetics
0982: Immunology
Identifier / keyword
Health and environmental sciences; Biological sciences; Recombinant DNA technology
Title
Cloning of a sialyltransferase gene to analyze surface sialic acid effects on metastasis
Author
Jain, Miten
Number of pages
191
Publication year
2008
Degree date
2008
School code
0656
Source
MAI 47/01M, Masters Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9780549729921
Advisor
Rittenhouse-Olson, Kate
University/institution
State University of New York at Buffalo
Department
Biotechnical & Clinical Lab Science
University location
United States -- New York
Degree
M.S.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
1456901
ProQuest document ID
304381398
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/304381398
Access the complete full text

You can get the full text of this document if it is part of your institution's ProQuest subscription.

Try one of the following:

  • Connect to ProQuest through your library network and search for the document from there.
  • Request the document from your library.
  • Go to the ProQuest login page and enter a ProQuest or My Research username / password.