Abstract/Details

Impact of microdialysis probes on vasculature and dopamine in the rat striatum: A combined fluorescence and voltammetric study and the design and optimization of a glutamate sensor to be used under hypoxic conditions


2008 2008

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Abstract (summary)

Measuring extracellular dopamine in the brain of living animals by means of microdialysis and/or voltammetry is a route towards understanding both normal brain function and pathology. Previous reports, however, suggest that the tissue response to implantation of devices may affect the outcome of the measurements. To address the source of the tissue response and its impact on striatal dopamine systems microdialysis probes were placed in the striatum of anesthetized rats. Images obtained by dual-label fluorescence microscopy show signs of ischemia, or reduced blood flow, and opening of the blood-brain barrier near the probe tracks. Opening of the blood-brain barrier was further examined by determining dialysate concentrations of carbi-DOPA, a drug that normally does not penetrate the brain. Although carbi-DOPA was recovered in brain dialysate, it did not alter dialysate dopamine levels or evoked dopamine release as measured by voltammetry near the probes. Microdialysis probes also significantly diminished the effect of intrastriatal infusion of kynurenate on extracellular dopamine levels as measured by voltammetry near the probes.

Glutamate is a very important neurotransmitter in the central nervous system. It is implicated in diseases such as schizophrenia, epilepsy, and stroke. Obtaining accurate information on glutamate concentrations is important in order to understand brain functioning. Glutamate is also of primary focus in terms of understanding the effects of hypoxia on the brain. It is believed that the large release of glutamate in the absence of oxygen is what is causing the toxic effects of hypoxia. To date, sensors used to monitor glutamate required oxygen to be present. Here, the design and optimization of a glutamate sensor to be used under hypoxic conditions is presented.

Indexing (details)


Subject
Physiology;
Analytical chemistry
Classification
0433: Physiology
0486: Analytical chemistry
Identifier / keyword
Pure sciences; Biological sciences; Brain vasculature; Dopamine; Glutamate sensor; Microdialysis; Platinum electrodes; Striatum
Title
Impact of microdialysis probes on vasculature and dopamine in the rat striatum: A combined fluorescence and voltammetric study and the design and optimization of a glutamate sensor to be used under hypoxic conditions
Author
Mitala, Christina Marie
Number of pages
118
Publication year
2008
Degree date
2008
School code
0178
Source
DAI-B 69/11, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9780549894797
University/institution
University of Pittsburgh
University location
United States -- Pennsylvania
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3335796
ProQuest document ID
304507389
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/304507389
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