Abstract/Details

The experimental evolution of host adaptation of the emerging pathogen <i>Burkholderia cenocepacia</i>


2008 2008

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Abstract (summary)

I investigated the ability of Burkholderia cenocepacia, an opportunistic bacterial pathogen, to adapt to a host. Studies have identified trade-offs associated with environmental adaptation, but few have investigated host adaptation. Consequently, I studied effects of adaptation by B. cenocepacia to onions (Allium cepa) on the ability to kill Caenorhabditis elegans. I hypothesized that adaptation to onions would reduce virulence in C. elegans. I evolved twelve populations of bacteria in onion tissue medium for 500 generations. Then, I quantified fitness differences between evolved and ancestral populations by direct competition, having developed molecular marking techniques to discriminate among competitors. Competitions revealed fitness increases in nine populations. Next, I measured virulence against C. elegans of each population and observed a reduced worm killing ability. I also quantified pleiotropic effects of adaptation related to virulence. In conclusion, I supported that adaptation of B. cenocepacia to one host resulted in decreased virulence in another host.

Indexing (details)


Subject
Genetics;
Microbiology
Classification
0369: Genetics
0410: Microbiology
Identifier / keyword
Biological sciences
Title
The experimental evolution of host adaptation of the emerging pathogen <i>Burkholderia cenocepacia</i>
Author
Ellis, Crystal Nicole
Number of pages
94
Publication year
2008
Degree date
2008
School code
0141
Source
MAI 46/05M, Masters Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9780549552086
Advisor
Cooper, Vaughn S.
University/institution
University of New Hampshire
University location
United States -- New Hampshire
Degree
M.S.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
1454999
ProQuest document ID
304524913
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/304524913
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