Abstract/Details

NMR backbone chemical shift assignments of the <i>hv</i>DHFR1:NADPH binary complex


2008 2008

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Abstract (summary)

Extremophiles are the organisms that survive in environments which are inhospitable to other creatures. This thesis made an attempt to understand the enviromental effects, in particular saline, on enzyme structure by using three dimensional NMR. The enzyme DHFR1 from halophile Haloferaxi volcanii is complexed with its cofactor NADPH, and the saline effects on the complex are studied by comparison with its apoenzyme, hvDHFR1. Backbone chemical shift assignments of the hvDHFR1:NADPH complex were attained which can be functional (along with future work) in understanding the effect of salts on enzyme structure, function, and flexibility. A total of 27 amino acids were found to show a significant change upon binding of NADPH and their positions were identified on enzyme complex. The secondary structure of hvDHFR1:NADPH is also predicted and overall global structure is found to be similar with the crystal structure of hvDHFR1 with few changes.

Indexing (details)


Subject
Chemistry;
Analytical chemistry;
Biochemistry
Classification
0485: Chemistry
0486: Analytical chemistry
0487: Biochemistry
Identifier / keyword
Pure sciences; Backbone chemical shifts; NADPH; NMR; hvDHFR1
Title
NMR backbone chemical shift assignments of the <i>hv</i>DHFR1:NADPH binary complex
Author
Vangala, Karthikeshwar
Number of pages
107
Publication year
2008
Degree date
2008
School code
0132
Source
MAI 47/02M, Masters Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9780549893028
Advisor
Young, John K.
Committee member
Armbrust, Kevin L.; Saebo, Svein
University/institution
Mississippi State University
Department
Chemistry
University location
United States -- Mississippi
Degree
M.S.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
1459798
ProQuest document ID
304529602
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/304529602
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