Effect of oxidative stress on histidine containing dipeptides, conjugated linoleic acid and alpha-tocopherol in animal and human muscle
Skeletal muscle contains a number of endogenous antioxidants that aid in protecting the muscle from oxidative damage. Antioxidant systems are comprised of water and lipid soluble compounds. The histidine containing dipeptides, anserine and carnosine, and α-tocopherol represent water and lipid soluble antioxidants which protect the muscle from oxidative damage. Raw and cooked turkey thigh and breast muscle were oxidatively challenged in order to understand the role of water and lipid soluble antioxidants in protecting the muscle from oxidative damage. The data suggest both water soluble and lipid soluble antioxidants affect the oxidative stability of turkey muscle.
Conjugated linoleic acid (CLA) was supplemented in the diets of rats in order to ascertain the antioxidant capacity of rat liver microsomes and muscle homogenates after up to 48 hr oxidation. CLA was shown to alter the fatty acid profile but was not shown to be as effective as α-tocopherol.
Trained and untrained muscle biopsies were oxidatively stressed for 3 hrs. Trained muscle homogenates exhibited lower hexanal formation and reduced α-tocopherol loss. Supplementation of untrained subjects failed to improve the oxidative stability of the muscle.
0307: Molecular biology