Abstract/Details

Trace element analysis and speciation of selenium, arsenic and gold in biological materials


2008 2008

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Abstract (summary)

Methods for determination and speciation of inorganic and organic selenium in complex matrices involving, inductively-coupled plasma mass spectrometry (ICP-MS), inductively-coupled plasma atomic emission spectrometry (ICP-AES) and high performance liquid chromatography (HPLC) were developed. Selenium species were separated and detected by mixed-mode weak anion exchange and reversed-phase stationary phases with ICP-MS and ICP-AES detection.

Selenium-containing nutritional supplements, wheat flour and selenized yeast, were analyzed and the major selenium species were identified and quantified by HPLC-ICP-MS equipped with a dynamic reaction cell (DRC).

The quantification response factors for different selenium and sulfur compounds, analyzed by DRC-ICP-MS and ICP-AES were determined. Possible reasons for the obtained results were discussed.

The effects of arsenic and selenium added simultaneously to the growth media of Helianthus annus (sunflowers), red sunflowers and Eichhornia crassipes (water hyacinths) were determined with respect to accumulation and metabolism. Total selenium and arsenic in shoots and roots was determined. Quantification of the selenium and arsenic species in E. crassipes by HPLC-ICP-MS and HPLC-ICP-AES was also performed.

An accurate and reliable method was developed for the determination of functionalized gold nanoparticles in hepatocarcinoma cell tissues and rat brain material by ICP-MS. A comparison between the gold accumulated in cell and brain tissues treated with neutral, positive and negative functionalized gold nanoparticles was performed.

Indexing (details)


Subject
Analytical chemistry
Classification
0486: Analytical chemistry
Identifier / keyword
Pure sciences; Arsenic; Gold nanoparticles; HPLC; ICP-MS; Selenium
Title
Trace element analysis and speciation of selenium, arsenic and gold in biological materials
Author
Dodova, Elena N.
Number of pages
220
Publication year
2008
Degree date
2008
School code
0118
Source
DAI-B 69/12, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9780549915188
Advisor
Tyson, Julian F.; Uden, Peter C.
Committee member
Parkash, Om; Voigtman, Edward G.
University/institution
University of Massachusetts Amherst
Department
Chemistry
University location
United States -- Massachusetts
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3336932
ProQuest document ID
304565843
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/304565843
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