Abstract/Details

Complexation of polyelectrolytes


2008 2008

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Abstract (summary)

Complexation found in nature was the inspiration and motivation to study three model systems to gain understanding into the underlying parameters that govern these events. Static and dynamic light scattering was predominately used to understand the complexation in three model systems: complexation of antimicrobial polymers with biomimetic vesicles, the complexation of protein to a semi-flexible polyelectrolyte and with a flexible polyelectrolyte.

Characterization of antimicrobial polymers in solution and their interactions with biomimetic vesicles were investigated in order to understand how antimicrobial polymers interacted with and killed bacteria. These studies observed that an aggregation of the vesicles correlated with antimicrobial activity. For these synthetic polymer systems, aggregation appeared to be a necessary component for antimicrobial activity,but was not indicative of activity.

Inspired by complexation found in nature between DNA and RNA and proteins model polyelectrolyte-protein systems were also investigated. The focus of this section was to understand how polymer flexibility, concentration, protein concentration, and ionic strength affected the phase behavior and presence of soluble aggregates in solution. Construction of phase diagrams for both semi-flexible and flexible polyelectrolye systems dsDNA and hyaluronic acid showed different phase diagrams,yet amazingly both systems showed a spontaneous selection of size of ∼230nm away from any phase boundary and was irrespective of salt concentration, polymer concentration, persistence length or protein concentration.

It was possible to gain insight into the internal packing of these two polyelectrolyte-protein complexes through static light scattering and fractal dimension analysis. Comparisons of the fractal dimension analysis of the DNA-lysozyme and HA-lysozyme was not affected by salt concentration and from analysis of the fractal dimension it was observed DNA-lysozyme aggregates, had a denser aggregate structure than the HA-lysozyme aggregate. It was also observed that away from the phase boundaries in each system the aggregate sizes and fractal dimensions were irrespective of polymer, salt, persistence length or protein concentration.

Indexing (details)


Subject
Polymer chemistry;
Condensed matter physics;
Biophysics
Classification
0495: Polymer chemistry
0611: Condensed matter physics
0786: Biophysics
Identifier / keyword
Pure sciences; Biological sciences; Antimicrobial polymers; Complexation; Dynamic light scattering; Polyelectrolytes
Title
Complexation of polyelectrolytes
Author
Pool, Joanna G.
Number of pages
130
Publication year
2008
Degree date
2008
School code
0118
Source
DAI-B 69/09, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9780549789307
Advisor
Muthukumar, Murugappan
Committee member
Coughlin, E. Bryan; Martin, Craig T.; Tew, Gregory N.
University/institution
University of Massachusetts Amherst
Department
Polymer Science & Engineering
University location
United States -- Massachusetts
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3325254
ProQuest document ID
304565970
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/304565970
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