Abstract/Details

Interface of protein-nanoparticle hybrid structures and self assembly of nanoparticles


2008 2008

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Abstract (summary)

Synthetic molecules mimicking protein surface properties have wide applications in nanobiotechnology. This thesis describes the impact of nanoparticles on important biological events such as control of protein-protein and protein-small molecule interactions. Nanoparticles tailored with organic ligands would selectively recognize cytochrome c (Cyt c) or cytochrome c peroxidase (CCP). They inhibited proteins mutual binding. The binding affinity of nanoparticles and structural properties of both proteins was studied. Moreover, binding interface between nanoparticles and proteins was determined by hydrogen/deuterium exchange mass spectrometry. Orientation of Cyt c on the nanoparticles can be altered by combination of hydrophobics and Coulombic on the monolayer. Furthermore, nanoparticles would affect the rate of electron transfer between Cyt c and small inorganic complex molecules, indicating that monolayer of particle play a key role on protein reactivity. The hemin coordination in CCP was unaffected by nanoparticle binding, indicating that monolayer is important for recognizing proteins as well as keeping proteins in native and fully active form. The CCP reactivity with small substrates was altered by nanoparticles, indicating unconventional approach to genetics for studying protein-substrate interactions.

In addition, self assembly of nanoparticles was studied using alternative Cyt c structure, indicating that loss of tertiary structure is critical for the formation of nanocomposite structures. The dependence of nanocomposite structure size to Cyt c:nanoparticle ratio and reversible formation of hybrid structures were discussed.

Indexing (details)


Subject
Chemistry
Classification
0485: Chemistry
Identifier / keyword
Pure sciences; Cytochrome c; Cytochrome c peroxidase; Self-assembly
Title
Interface of protein-nanoparticle hybrid structures and self assembly of nanoparticles
Author
Bayraktar, Halil
Number of pages
184
Publication year
2008
Degree date
2008
School code
0118
Source
DAI-B 69/12, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9780549916031
Advisor
Knapp, Michael J.
Committee member
Rotello, Vincent M.; Sun, Lianhong; Weis, Robert M.
University/institution
University of Massachusetts Amherst
Department
Chemistry
University location
United States -- Massachusetts
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3337016
ProQuest document ID
304566882
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/304566882
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