Abstract/Details

A post-import pathway for protein targeting to chloroplast inner membrane


2008 2008

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Abstract (summary)

The chloroplast envelope plays critical roles in the synthesis and regulated transport of key metabolites, including intermediates in photosynthesis and lipid metabolism. Despite this importance, the biogenesis of the envelope membranes has not been investigated in detail. To identify the determinants of protein targeting to the inner envelope membrane (IM), I investigated the targeting of the nucleus-encoded integral IM protein, atTic40. I found that pre-atTic40 is imported into chloroplasts and processed to an intermediate size (int-atTic40) before insertion into the IM. Int-atTic40 is soluble and inserts into the IM from the internal stromal compartment. I also show that atTic40 and a second IM protein, atTic110, can target and insert into isolated IM vesicles in vitro. These in vitro studies are further supported by in vivo evidence showing pre-atTic40 directly engineered into the chloroplast genome and expressed within chloroplasts can efficiently target to the inner envelope membrane. Taken together, my experiments are consistent with a "post import" mechanism in which the IM proteins are first imported from the cytoplasm and subsequently inserted into the IM from the stroma.

Indexing (details)


Subject
Cellular biology
Classification
0379: Cellular biology
Identifier / keyword
Biological sciences; Chloroplasts; Inner membranes
Title
A post-import pathway for protein targeting to chloroplast inner membrane
Author
Li, Ming
Number of pages
116
Publication year
2008
Degree date
2008
School code
0118
Source
DAI-B 69/12, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9780549915362
Advisor
Schnell, Danny
Committee member
Bezanilla, Magdalena; Hebert, Daniel; Heuck, Alejandro
University/institution
University of Massachusetts Amherst
Department
Molecular & Cellular Biology
University location
United States -- Massachusetts
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3336950
ProQuest document ID
304569860
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/304569860
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