Abstract/Details

Genetic studies of the regulation of RecA activity in <i>Escherichia coli</i>: A two filament model


2008 2008

Other formats: Order a copy

Abstract (summary)

The RecA protein of Escherichia coli is a central enzyme in recombination and SOS induction, both crucial DNA repair pathways. Furthermore, RecA and its homologues are found in all domains of life, from bacteria to humans. Much of the understanding of RecA activity has been based on interpretations of in vitro studies. One underlying assumption in this work is that all RecA activities will behave identically in the capacity to catalyze recombination and induce the SOS response. However, our work has shown that this assumption is not valid when applied to RecA filaments formed in vivo. RecA filaments formed during exponential (log phase) growth are less active for SOS induction than those formed after UV irradiation.

In my work, I have attempted to better characterize the factors that regulate RecA activity in E. coli under these different conditions. These factors can be either properties of RecA itself or other proteins or cellular conditions. We have found that ATPase activity and DNA binding are two key factors of RecA that control activity. Furthermore, other proteins, such as RecX, DinI and RecF and RecB further modulate the activity of RecA. This work has lead to a better understanding of pathways found throughout eubacteria. This understanding has ramifications in diverse fields, including bacterial pathogenesis and human cancer research.

Indexing (details)


Subject
Genetics;
Microbiology
Classification
0369: Genetics
0410: Microbiology
Identifier / keyword
Biological sciences; DNA damage response; Escherichia coli; RecA; SOS induction
Title
Genetic studies of the regulation of RecA activity in <i>Escherichia coli</i>: A two filament model
Author
Renzette, Nicholas
Number of pages
205
Publication year
2008
Degree date
2008
School code
0118
Source
DAI-B 69/12, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9780549915133
Advisor
Sandler, Steven J.
Committee member
Holden, James F.; Pomposiello, Pablo J.; Riley, Margaret A.
University/institution
University of Massachusetts Amherst
Department
Molecular & Cellular Biology
University location
United States -- Massachusetts
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3336927
ProQuest document ID
304574180
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/304574180
Access the complete full text

You can get the full text of this document if it is part of your institution's ProQuest subscription.

Try one of the following:

  • Connect to ProQuest through your library network and search for the document from there.
  • Request the document from your library.
  • Go to the ProQuest login page and enter a ProQuest or My Research username / password.