Expression and processing of xanthin dehydrogenase/oxidase cDNA from African cape buffalo

2000 2000

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Abstract (summary)

Our lab studies oxidative regulation in defense against extracellular pathogens, especially African Trypanosomes.

Trypanosomes are protozoan hemoflagellates. The organisms are transmitted by tsetse flies (Golssina spp) and cause fatal trypanosomiasis in people and domestic animals. Previous work in our lab showed that African Cape buffalo and eland plasma contain a trypanocidal protein which kills all species of African trypanosomes during short term incubation in vitro (Black, 1999). The protein was identified as xanthine oxidase (Muranjan, 1997). On-going work in our lab has shown that the level of xanthine oxidase is a species and breed characteristic. The main focus of this investigation was to explore the molecular basis underlying the intrinsic systemic XO activity differences between different mammal species, with a view toward elucidating strategies that might enhance resistance of domestic animals to trypanosomiasis.

My work has shown that the systemic XO activity differences observed in different mammal species, including Cape buffalo, eland, cattle, human, rat and mouse, does not result form XO coding sequence variation. Neither does it result from differences in tissue expression or transcription efficiency. Xanthine oxidase was proposed as a cytosolic protein, lacking any known leader/targeting sequences. My work also showed that there was more intensive signal in the paranuclear region. Expression fragments of the XOR protein revealed unconventional targeting sequence residing in both the N-terminus and the C-terminus of the enzyme, and they all target the recombinant protein to the paranuclear region. Sequence analysis confirmed the presence of two nucleus targeting sequence, which seem to agree with the role of xanthine oxidase in oxidant-mediated signal transduction in ischemia/reperfusion injury. We also found that XOR was not secreted into the medium under standard tissue culture growth conditions, nor was it displayed on the cell surface. This strongly suggests that the XOR protein might not be transported into the circulatory system under normal physiological condition and the presence of XOR in plasma could simply result from its release during cell death as a result of normal cell turn-over.

Indexing (details)

Molecular biology
0307: Molecular biology
Identifier / keyword
Biological sciences; Cape buffalo; Syncerus caffer; Trypanosome; Xanthin dehydrogenase
Expression and processing of xanthin dehydrogenase/oxidase cDNA from African cape buffalo
Wang, Jun
Number of pages
Publication year
Degree date
School code
DAI-B 61/07, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
9780599845176, 0599845171
Black, Samuel J.
University of Massachusetts Amherst
University location
United States -- Massachusetts
Source type
Dissertations & Theses
Document type
Dissertation/thesis number
ProQuest document ID
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
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