Abstract/Details

Tissue-targeted metabonomics: Metabolic profiling by microdialysis and NMR spectroscopy


2008 2008

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Abstract (summary)

This research offers an alternative approach to the study of tissue-specific metabolism by metabonomics, called tissue-targeted metabonomics. Microdialysis sampling was used to sample small endogenous metabolites in tissue extracellular fluid. Samples were then analyzed by proton nuclear magnetic resonance spectroscopy. The research explored analytical and biological considerations for this method. Data treatment, including integration of the NMR spectra and normalization to standards, was optimized for different dialysate types. Time trends in the basal metabolism of liver and heart tissues were described, as were the metabolic effects of anesthesia, circadian rhythms and animal activity. After the method was developed and basal metabolism was adequately characterized, tissue-targeted metabonomics was used to study oxidative stress, induced locally by perfusing doxorubicin through a probe in the liver. Results indicated a localized metabolic response to the doxorubicin dose in awake rats, illustrating the potential of tissue-targeted metabonomics to monitor localized metabolic changes for many applications.

Indexing (details)


Subject
Analytical chemistry
Classification
0486: Analytical chemistry
Identifier / keyword
Pure sciences; Metabolic profiling; Metabonomics; Microdialysis; NMR; Oxidative stress
Title
Tissue-targeted metabonomics: Metabolic profiling by microdialysis and NMR spectroscopy
Author
Price, Kristin Elizabeth
Number of pages
257
Publication year
2008
Degree date
2008
School code
0099
Source
DAI-B 70/03, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9781109062953
Advisor
Lunte, Craig E.
Committee member
Desaire, Heather; Dunn, Bob; Larive, Cindy K.; Laurence, Jennifer
University/institution
University of Kansas
Department
Chemistry
University location
United States -- Kansas
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3349943
ProQuest document ID
304616375
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/304616375
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