Abstract/Details

Genetic regulation of collective cell migration


2008 2008

Other formats: Order a copy

Abstract (summary)

Cell migration is a characteristic feature of numerous biologically important processes such as embryo development, tissue homeostasis, wound healing, inflammation, and cancer metastasis. The migration of cells in groups, known as collective cell migration, is observed during embryogenesis and dissemination of some types of cancers. Many relevant aspects of collective cell migration remain unanswered: how is the migratory group selected from the larger population of cells from which they derive? How are the adhesive contacts among the members of the collective regulated during migration? What are the molecular mechanisms that cells use to migrate in groups? We have used the collective movements that occur during Drosophila oogenesis as a genetically tractable system to answer such questions.

The gene apontic was identified in a screen for genes that affect border cell migration. apontic mutant egg chambers contain 2-7 extra-migratory cells. We found apontic to be a transcriptional target of STAT and a negative regulator of STAT activity. Therefore, apontic is required to limit the migratory cell population by transforming a graded pattern of STAT activation into a step-wise cell fate decision.

Hindsight is a transcription factor required during morphogenetic changes during Drosophila embryogenesis. Anterior follicle cells mutant for hindsight failed to flatten and border cells and failed to migrate. hindsight regulates several adhesion proteins. Down-regulation of the hindsight human homolog RREB1 restricted migration of breast epithelial cells. Altogether, hindsight adjusts the levels of adhesion contacts among the members of a migratory group to allow morphogenesis while maintaining tissue integrity.

Border cells appear to undergo a partial epithelial to mesenchymal transition (EMT) as they migrate. We are investigating the role of snail, a regulator of EMT, during border cell migration. snail mutant border cells failed to initiate migration and remained within the follicular epithelium. snail over-expressing border cells also showed severe migration defects. Low levels of snail expression might therefore be required to induce a partial-EMT in the border cells and promote migration.

The genes studied in this Dissertation, fine-tune different and equally important aspects of collective migration: selection of the migratory population, regulation of adhesion strength and partial EMT.

Indexing (details)


Subject
Genetics;
Cellular biology;
Biochemistry
Classification
0369: Genetics
0379: Cellular biology
0487: Biochemistry
Identifier / keyword
Pure sciences; Biological sciences; Apontic; Cell migration; Drosophila; Hindsight; STAT
Title
Genetic regulation of collective cell migration
Author
Melani, Mariana
Number of pages
170
Publication year
2008
Degree date
2008
School code
0098
Source
DAI-B 69/04, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9780549581079
Advisor
Montell, Denise
University/institution
The Johns Hopkins University
University location
United States -- Maryland
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3309722
ProQuest document ID
304638391
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/304638391
Access the complete full text

You can get the full text of this document if it is part of your institution's ProQuest subscription.

Try one of the following:

  • Connect to ProQuest through your library network and search for the document from there.
  • Request the document from your library.
  • Go to the ProQuest login page and enter a ProQuest or My Research username / password.