Abstract/Details

Gene expression and development in blowflies: A method to more precisely estimate postmortem intervals


2007 2007

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Abstract (summary)

The use of insects in forensic entomology has become one of the most helpful tools for estimating post mortem interval (PMI). Within minutes of death, Calliphoridae flies colonize human corpses and are the most accurate to estimate PMI. Currently, length measurements of larvae combined with environmental temperature, as well as the number of posterior spiracles present are used to estimate the developmental stage of maggots. There are many drawbacks to using these methods for PMI determination, including shrinkage of larvae as the third instar ends and variation among growth rates. Therefore, alternative methods are needed to increase the precision of PMI estimation. Molecular studies, especially those including Drosophila melanogaster, have helped scientists understand gene expression and regulation. Throughout the life cycle of a fly, thousands of genes are up- and down-regulated, thus if one could determine the level at which a gene is expressed it may provide an estimation of age. The third instar is the longest and most difficult stage in Lucilia sericata to age, therefore ideal for gene expression analysis. Six genes were examined in the insect including ADP/ATP translocase, cuticle 1, chitin synthase, chymotrypsinogen, alpha trypsin, and aminopeptidase. Based on the research presented here, they show unique patterns that can be used to estimate larval development.

Indexing (details)


Subject
Molecular biology;
Forensic anthropology;
Entomology;
Criminology
Classification
0307: Molecular biology
0339: Forensic anthropology
0353: Entomology
0627: Criminology
Identifier / keyword
Social sciences; Biological sciences
Title
Gene expression and development in blowflies: A method to more precisely estimate postmortem intervals
Author
Jennings, Kimberly C.
Number of pages
68
Publication year
2007
Degree date
2007
School code
0128
Source
MAI 46/02M, Masters Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9780549251446
Advisor
Foran, David R.
University/institution
Michigan State University
University location
United States -- Michigan
Degree
M.S.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
1448480
ProQuest document ID
304851350
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/304851350
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