Abstract/Details

Development of novel microfluidic cell sorters using dielectrophoresis and magnetophoresis


2009 2009

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Abstract (summary)

Microfluidics offers an attractive platform to separate biological components out of a complex mixture at high purity, which is indispensable in biotechnology. This dissertation describes three novel microfluidic techniques which achieve separation by exploiting dielectrophoresis and magnetophoresis. It describes unique approaches in utilizing microfluidic technology for advanced applications in cellular and molecular separations, and demonstrates novel and useful functionalities are difficult to implement using conventional, macro-microscale methods.

First, I report a microfluidic device that utilizes the dielectrophoresis phenomenon to synchronize cells by exploiting the relationship between the cell’s volume and its phase in the cell cycle. The dielectrophoresis activated cell synchronizer (DACSync) device accepts an asynchronous mixture of cells at the inlet, fractionates the cell populations according to the cell-cycle phase (G1/S and G2/M), and elutes them through different outlets. The device is gentle and efficient; it utilizes electric fields that are 1–2 orders of magnitude below those used in electroporation and enriches asynchronous tumor cells in the G1 phase to 96% in one round of sorting, in a continuous flow manner at a throughput of 2×105 cells per hour per microchannel.

Second, I describe a microfluidic platform to sort out multiple cell types simultaneously with high purity and recovery. I describe a novel approach to specifically label multiple cell types with unique synthetic dielectrophoretic tags that modulate the complex permittivities of the labeled cells, allowing them to be sorted with high purity using the multitarget dielectrophoresis activated cell sorter (MT-DACS) chip. Here I describe the underlying physics and design of the MT-DACS microfluidic device and demonstrate ∼1000-fold enrichment of multiple bacterial target cell types in a single-pass separation.

Finally, I further expand our multi-target cell sorter scheme and report the development of integrated Dielectrophoretic-Magnetic Activated Cell Sorter (iDMACS), which is, to the best of our knowledge, the first platform to integrate two different force fields in a single microfluidic device for highly efficient multi-target separation. I describe the underlying physics and design of the iDMACS and demonstrate ∼900-fold enrichment of multiple bacterial target cell types with over 95% purity after a single round of separation.

Indexing (details)


Subject
Biomedical engineering;
Electrical engineering;
Mechanical engineering
Classification
0541: Biomedical engineering
0544: Electrical engineering
0548: Mechanical engineering
Identifier / keyword
Applied sciences; BioMEMS; Cell sorting; Dielectrophoresis; Lab-on-a-chip; Magnetophoresis; Microfluidics
Title
Development of novel microfluidic cell sorters using dielectrophoresis and magnetophoresis
Author
Kim, Unyoung
Number of pages
108
Publication year
2009
Degree date
2009
School code
0035
Source
DAI-B 70/09, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9781109329612
Advisor
Soh, Hyongsok T.
Committee member
Daugherty, Patrick S.; Meinhart, Carl D.; Turner, Kimberly L.
University/institution
University of California, Santa Barbara
Department
Mechanical Engineering
University location
United States -- California
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3371653
ProQuest document ID
304851460
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/304851460
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