Abstract/Details

Specificity of inhibitory control of cortical interneurons in layer 4 of mouse somatosensory barrel cortex


2009 2009

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Abstract (summary)

Cortical processing of sensory information relies on a diversity of inhibitory control. This thesis examines the cell type specificity in layer 4 of mouse somatosensory barrel cortex of two understudied mechanisms of GABA inhibition: (1) tonic inhibition mediated by specific GABAA receptors, and (2) GABAB receptor mediated inhibition. Using whole-cell patch clamp techniques, we find that tonic GABAA inhibition is greatest in inhibitory neurons. This inhibition of inhibitory neurons produces a net excitatory effect. Within inhibitory neurons, there is additional diversity of tonic GABAA inhibition, with consequences for information processing, seen specifically in fast spiking (FS) mediated feed forward inhibition. GABA B receptor mediated inhibition of neurotransmitter release acts on both excitatory and inhibitory neurons, with the greatest effect seen in a non-FS interneurons. Paired recordings between synaptically coupled neurons show an approximate 50% inhibition at synapses with either excitatory or FS inhibitory interneurons presynaptic, while responses produced by non-FS cells were essentially abolished by activation of GABAB receptors. In contrast to tonic GABAA inhibition, in adult animals, inhibitory currents from activation of GABAB receptors is greatest in excitatory neurons, rather than inhibitory neurons. However, in younger animals, there is equal GABA B inhibition of non-FS inhibitory interneurons. This inhibition decreases with age, with potential implications for plasticity.

Indexing (details)


Subject
Neurosciences
Classification
0317: Neurosciences
Identifier / keyword
Biological sciences; Cortical interneurons; Electrophysiology; G-protein coupled receptors; GABA(A); GABA(B); Inhibitory control; Somatosensory; Somatosensory barrel cortex; Tonic
Title
Specificity of inhibitory control of cortical interneurons in layer 4 of mouse somatosensory barrel cortex
Author
Krook-Magnuson, Esther
Number of pages
96
Publication year
2009
Degree date
2009
School code
0076
Source
DAI-B 70/05, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9781109157567
Advisor
Huntsman, Molly M.
Committee member
Ahern, Gerard; Hales, Tim; Vicini, Stefano; Wu, Jian-Young
University/institution
Georgetown University
Department
Pharmacology
University location
United States -- District of Columbia
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3356049
ProQuest document ID
304887586
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/304887586/abstract
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