Abstract/Details

Retinoic acid transfer and protein-protein interaction between the cellular retinoic acid binding protein and the retinoic acid receptor probed by hydrogen deuterium exchange mass spectrometry


2009 2009

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Abstract (summary)

Retinoic acid (RA) is the most potent metabolite of vitamin A and is utilized in signaling pathways that control many cellular events. Cellular retinoic acid binding proteins I and II are carriers of RA inside the cell and CRABPII transports RA to the nucleus where it binds to the retinoic acid receptors (RARs) and can then exert its effect on the transcription of genes. CRABPI is thought to bind excess RA in the cell cytoplasm to protect it from the oxidizing effect of RA. The interaction between CRABPs and RARs is very transient and such a complex has never been observed. Entry and exit of RA in CRABP occurs through fluctuation of the protein backbone through the portal region. Based on this and the crystal structures of CRABPs, a mutant containing a disulfide bond in the portal region it the holo-form of the protein was designed. This mutant effectively “locks” RA inside its binding cavity in the presence of lipid vesicles, confirming that ligand movement in and out of CRABP necessitates backbone fluctuation. Using a combination of Bioaffinity chromatography and hydrogen deuterium exchange mass spectrometry, the mechanism of transfer of RA during the CRABP/RA/RAR interaction was studied. Holo-CRABP was more protected than the apo-form and the presence of RAR did not result in a change in the protection level of apo-CRABP. However, the exchange kinetics of holo-CRABP in the presence of RAR were different from that of apo- or holo-CRABP alone. The CRABP mutants containing a disulfide bond in the portal region have an initial exchange rate that is higher than for the wild type CRABP. The complexes between those holo-mutants and RAR have a longer half-life than the wild type CRABP. The accelerated hydrogen deuterium exchange kinetics of the holomutant in the presence of RAR could therefore not be the result of faster ligand transfer from CRABP to RAR. These results were observed with both CRABPI and CRABPII. This led to a model in which during RA transfer from CRABP to RAR, the backbone of CRABP is destabilized through its interaction with RAR.

Indexing (details)


Subject
Analytical chemistry;
Biochemistry
Classification
0486: Analytical chemistry
0487: Biochemistry
Identifier / keyword
Pure sciences; Cellular retinoic acid binding proteins; Hydrogen deuterium exchange; Protein-protein interactions; Retinoic acid; Retinoic acid receptors
Title
Retinoic acid transfer and protein-protein interaction between the cellular retinoic acid binding protein and the retinoic acid receptor probed by hydrogen deuterium exchange mass spectrometry
Author
Sjoelund, Virginie
Number of pages
145
Publication year
2009
Degree date
2009
School code
0118
Source
DAI-B 70/12, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9781109500967
Advisor
Kaltashov, Igor
Committee member
Eyles, Stephen; Hardy, Jeanne; Roberts, Susan
University/institution
University of Massachusetts Amherst
Department
Molecular & Cellular Biology
University location
United States -- Massachusetts
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3380022
ProQuest document ID
304920499
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/304920499
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