Abstract/Details

Applying surface modified gold nanoparticles to biological systems


2009 2009

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Abstract (summary)

Gold nanoparticles hold promise as both a stable drug delivery system as well as a targeted inhibitor of protein:protein interactions. The flexibility and ease of modifying the surface of nanoparticles enables them to be tailored to specific cellular environments and tasks. Due to these characteristics, I have been able to effectively modify gold.

To further investigate gold nanoparticles as drug delivery systems, it is important to understand where they localize once inside the cell. One possible way of determining the cellular fate of the nanoparticles is conduction cell fractionization studies coupled with ICP-MS. A protocol using sucrose gradients along with high-speed ultracentrifugation allows for the various cellular organelles to be separated and digested. Once each fraction is digested, I can monitor where the gold nanoparticles localize inside the cell.

Indexing (details)


Subject
Biochemistry;
Organic chemistry
Classification
0487: Biochemistry
0490: Organic chemistry
Identifier / keyword
Pure sciences; Chymotrypsin; Endosomes; Gold nanoparticles; HeLa cells; Protein:protein interactions
Title
Applying surface modified gold nanoparticles to biological systems
Author
Arvizo, Rochelle R.
Number of pages
69
Publication year
2009
Degree date
2009
School code
0118
Source
DAI-B 70/09, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9781109351965
Advisor
Rotello, Vincent M.
Committee member
Jerry, D. Joseph; Schnarr, Nathan A.; Thayumanavan, Sankaran
University/institution
University of Massachusetts Amherst
Department
Chemistry
University location
United States -- Massachusetts
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3372254
ProQuest document ID
304922848
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/304922848
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