Abstract/Details

Is organic tissue culture of petunia possible?


2009 2009

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Abstract (summary)

In vitro experiments were conducted using leaf explants from organically grown Petunia x hybrida "Blue Picotee" cultured on media prepared from organic hydroponic solutions, Murashigi and Skoog (MS) vitamins and benzyladenine. Explants cultured on MS regenerated the best. Explants cultured on Aqua Power produced shoots, had higher weights and lower necrosis than on other organic media. The Aqua Power results were not repeatable. In testing Aqua Power, Earth Juice and Peaceful Valley at ⅓, ⅔ and full concentrations, the explants on Aqua Power were 100% necrotic at all concentrations. Explants on Earth Juice and Peaceful Valley were chlorotic but had the best growth, measured by weight and necrosis, at the lowest concentration. Shoot organogenesis occurred on explants at all concentrations on Peaceful Valley. When using liquid petunia extract media, no shoots regenerated. On these media, the highest explant weights were with 7.5 g·L -1 agar and necrosis decreased as agar concentration increased.

Indexing (details)


Subject
Horticulture;
Plant sciences
Classification
0471: Horticulture
0479: Plant sciences
Identifier / keyword
Biological sciences; Micropropagation; Petunia; Propagation; Tissue culture
Title
Is organic tissue culture of petunia possible?
Author
Massa, Denise Marie
Number of pages
106
Publication year
2009
Degree date
2009
School code
0209
Source
MAI 47/06M, Masters Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9781109173079
Advisor
Preece, John E.
Committee member
Ebbs, Stephen D.; Henry, Paul H.
University/institution
Southern Illinois University at Carbondale
Department
Plant and Soil Science
University location
United States -- Illinois
Degree
M.S.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
1464977
ProQuest document ID
304997083
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/304997083
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