Abstract/Details

Molecular analysis of fetal nucleic acids for prenatal investigations


2004 2004

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Abstract (summary)

Prenatal investigations are an integral part of modern obstetrics care. Major advances in molecular genetics have led to the development of services, such as genetic counselling, prenatal and preimplantation diagnosis, that cater for the investigation of fetal genetic and chromosomal abnormalities. In recent years, extra demands have been placed on these services as a result of the emerging sociocultural trend to delay childbirth and to maintain smaller family units. However, current prenatal investigative programmes rely on fetal tissue sampling procedures such as amniocentesis and chorionic villus sampling for the provision of a confirmatory diagnosis. By virtue of their invasive nature, these procedures are associated with a risk of spontaneous abortion. Hence, there have been ongoing efforts to develop safer alternatives to conventional prenatal diagnostic procedures. This thesis focuses on the development of molecular approaches and protocols which facilitate the implementation of safer alternatives to the prenatal diagnostic methods in current clinical use. The recent discovery of the existence of fetal nucleic acids in plasma and serum of pregnant women has led to new exciting possibilities for the development of non-invasive prenatal diagnostic approaches. Hence, studies described in this thesis examine methods and protocols that would enhance the application of circulating fetal DNA detection for pregnancy monitoring or prenatal diagnosis. During the course of this work, a number of traditionally perceived challenges to circulating fetal nucleic acid analysis have been overcome. Lastly, the potential impact and implications of the work in this thesis are discussed and speculated.

Indexing (details)


Subject
Obstetrics;
Gynecology;
Biomedical research
Classification
0380: Obstetrics
0380: Gynecology
0541: Biomedical research
Identifier / keyword
Health and environmental sciences; Applied sciences; Fetal nucleic acids; Noninvasive testing
Title
Molecular analysis of fetal nucleic acids for prenatal investigations
Author
Chiu, Wai Kwun Rossa
Number of pages
177
Publication year
2004
Degree date
2004
School code
1307
Source
DAI-B 65/10, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9780496102327
Advisor
Lo, Y. M. Dennis
University/institution
The Chinese University of Hong Kong (Hong Kong)
University location
Hong Kong
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3150508
ProQuest document ID
305050703
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/305050703
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