Abstract/Details

A genomic approach to the study of chromatin and transcription


2004 2004

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Abstract (summary)

The eukaryotic cell has developed complex regulatory circuitry to ensure proper gene expression. An important aspect of this regulation involves the modulation of the basal transcriptional machinery as well as nucleosomal chromatin substrates. Both covalent histone modification and the physical movement or positioning of nucleosomes can impart regulatory functions on transcription. This work describes genomic approaches to study chromatin modification and how it affects transcription in the yeast Saccharomyces cerevisiae. Specifically, we define the in vivo DNA targets of the ATP-dependent chromatin remodeler RSC (Remodels the Structure of Chromatin). We also examine the recruitment and regulation of the basal machinery for RNA polymerase III. Finally, we provide evidence for novel transcriptional roles for the histone acetyltransferase complexes SAGA (Spt-Ada-Gcn5-Acetyltransferase) and Elongator. These studies have provided a better understanding of the relationships between chromatin and gene regulation.

Indexing (details)


Subject
Oncology;
Molecular biology;
Genetics
Classification
0992: Oncology
0307: Molecular biology
0369: Genetics
Identifier / keyword
Health and environmental sciences; Biological sciences; Chromatin; RNA polymerase III; RSC; Transcription
Title
A genomic approach to the study of chromatin and transcription
Author
Roberts, Douglas N.
Number of pages
128
Publication year
2004
Degree date
2004
School code
0240
Source
DAI-B 65/12, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9780496165346, 0496165348
Advisor
Cairns, Brad
University/institution
The University of Utah
University location
United States -- Utah
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3155933
ProQuest document ID
305101694
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/305101694
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