Abstract/Details

Complex polysaccharide degradation by <i>Microbulbifer degradans</i> strain 2-40: Studies of the chitinolytic system and carbohydrase architecture


2004 2004

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Abstract (summary)

Microbulbifer degradans strain 2-40 is a Gram negative marine bacterium that is able to depolymerize and metabolize a wide variety of complex polysaccharides, including chitin. Chitin is the second most abundant biopolymer in nature and is a widely available nutrient in many environments. The chitinolytic system of M. degradans consists of three chitin depolymerases (ChiA, ChiB, and ChiC), three N-acetylglucosaminidases (HexA, HexB, and HexC), a chitodextrinase (CdxA), a chitin-binding protein (CbpA), and a suite of enzymes involved in the transport and metabolism of GlcNAc and GlcNAc2. ChiB, the largest eubacterial chitinase described, includes two complete Glycoside Hydrolase family 18 catalytic domains; one is exolytic and the other is endolytic. These catalytic domains share similar reaction optima, temperature, pH, and metal ion sensitivities, and are shown to function synergistically in the depolymerization of crystalline chitin. ChiA, ChiB, and 44 other M. degradans carbohydrases contain polyserine linker regions. These linkers are composed predominantly of serine (79%), have an average length of 39 residues, and are encoded by all six serine codons without any obvious bias or pattern. Polyserine domains are found only between functional groups (e.g., catalytic, binding, or anchoring domains) and are only found in putative secreted, carbohydrate depolymerases. The sequence of the M. degradans genome was determined during the course of this work and permitted the first genomic analysis of a chitinolytic organism to be performed. Further, these experiments and subsequent analyses have lead to a greater understanding of how M. degradans is able to metabolize such a diverse collection of biopolymers.

Indexing (details)


Subject
Microbiology;
Molecular biology
Classification
0410: Microbiology
0307: Molecular biology
Identifier / keyword
Biological sciences; Carbohydrase; Chitinolytic; Polysaccharide
Title
Complex polysaccharide degradation by <i>Microbulbifer degradans</i> strain 2-40: Studies of the chitinolytic system and carbohydrase architecture
Author
Howard, Michael B.
Number of pages
222
Publication year
2004
Degree date
2004
School code
0117
Source
DAI-B 65/11, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9780496126323, 0496126326
Advisor
Hutcheson, Steven W.
University/institution
University of Maryland, College Park
University location
United States -- Maryland
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3152311
ProQuest document ID
305178266
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/305178266
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