Abstract/Details

Anaerobic degradation of 2,4,6-trinitrotoluene (TNT): Molecular analysis of active degraders and metabolic pathways


2010 2010

Other formats: Order a copy

Abstract (summary)

Nitroaromatic compounds have been historically used as dyes, explosives and pesticides. The disposal of these products has caused widespread contamination of both soil and groundwater. 2,4,6-trinitrotoluene (TNT) has been known as the worst of these contaminants because of the mutagenic properties of the compound, its persistence in the environment, and the wide range of sites that are contaminated with TNT. It is not only harmful to humans but it is also harmful to organisms in the lower trophic levels of ecosystems, and can affect the primary production of phytoplankton in the oceans.

The following series of experiments looks to determine ways that 2,4,6-trinitrotoluene could be biologically degraded in contaminated anaerobic environments. The experiments use dilution culturing, molecular techniques, and chemical analysis. Cultures were made from 3 different geographical sites (Arthur Kill, Norfolk Harbor, and an unexploded ordnance site in Hawaii) under both sulfidogenic and methanogenic conditions. The experiments demonstrate that there are bacteria present in the environment that could degrade TNT and the use of stable-isotope probing (SIP) in dilution cultures allowed the bacteria that are able to initially degrade TNT to be identified. The stable-isotope fed dilution cultures produced samples that were used for chemical analysis to determine pieces of the biological degradation pathway. These experiments determined that (1) there are bacteria that are able to utilize both the carbon and/or nitrogen present in TNT for growth and (2) toluene, methylphloroglucinol, benzoate or a benzoate derivative, and a cresol are all metabolites of anaerobic TNT degradation.

Indexing (details)


Subject
Microbiology;
Environmental science
Classification
0410: Microbiology
0768: Environmental science
Identifier / keyword
Health and environmental sciences; Biological sciences; Anaerobic degradation; Metabolic pathways; Stable-isotope probing; Trinitrotoluene
Title
Anaerobic degradation of 2,4,6-trinitrotoluene (TNT): Molecular analysis of active degraders and metabolic pathways
Author
Gallagher, Erin Maureen
Number of pages
127
Publication year
2010
Degree date
2010
School code
0190
Source
DAI-B 71/04, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9781109692211
Advisor
Young, Lily
University/institution
Rutgers The State University of New Jersey - New Brunswick
Department
Graduate School - New Brunswick
University location
United States -- New Jersey
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3397376
ProQuest document ID
305225555
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/305225555
Access the complete full text

You can get the full text of this document if it is part of your institution's ProQuest subscription.

Try one of the following:

  • Connect to ProQuest through your library network and search for the document from there.
  • Request the document from your library.
  • Go to the ProQuest login page and enter a ProQuest or My Research username / password.