Abstract/Details

Interactions between Uba80 and Gag during the assembly of human immunodeficiency virus type 1


2010 2010

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Abstract (summary)

HIV-1 assembly and budding are promising targets for drug design. Gag, the core viral structural protein, requires the aid of host machinery during assembly and egress. Ubiquitination of Gag is proposed to be involved, but its role remains obscure. Here we study ubiquitin derived from one of four human loci, Uba80. Uba80 encodes two proteins, a mono-ubiquitin and Rps27a. We show that ubiquitin, expressed alone or in the context of full-length Uba80 conjugates to many proteins in the viral particle and that the majority of ubiquitin is neither conjugated to Gag nor free, likely linked to host factors. We demonstrate that the C-terminally encoded ubiquitin “extension protein” Rps27a, a small subunit ribosomal protein, interacts with Gag and is packaged into virus particles. In localization studies, overexpression of Uba80 redistributes Gag to the nucleolus. These data suggest that Gag may interact with both Ubiquitin conjugated proteins and Rps27a during assembly.

Indexing (details)


Subject
Virology
Classification
0720: Virology
Identifier / keyword
Biological sciences; Gag; HIV-1; Rps27a; S27a; Uba80; Ubiquitin
Title
Interactions between Uba80 and Gag during the assembly of human immunodeficiency virus type 1
Author
Faust, Jeremy
Number of pages
44
Publication year
2010
Degree date
2010
School code
1353
Source
MAI 48/05M, Masters Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9781109748932
Advisor
Chen, Benjamin K.
Committee member
Blitzer, Robert; Chang, Theresa; Ting, Adrian
University/institution
Mount Sinai School of Medicine of New York University
Department
Immunology
University location
United States -- New York
Degree
M.S.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
1475604
ProQuest document ID
305242897
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/305242897
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