Abstract/Details

A new biocompatible coating for bioanalytical devices based on PSI (polysuccinimide)


2006 2006

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Abstract (summary)

Protein chips need to immobilize proteins that retain their native structures. Polymer coatings can be used to build up nanostructures on a protein-chip. This is generally done by synthesizing the polymer with a series of steps. Polymer coatings reported in this dissertation were prefabricated in solution as small polymers and then assembled into a coating in a single, or a small number of steps. This reduces the time and the complexity required for device fabrication such as microfluidic systems.

Prefabricated polysuccinimide (PSI) coatings were developed as a new generation of biocompatible coatings for microfabricated analytical systems. These coatings were developed for gold and/or glass BioCD discs. Linear and branched PSIs were characterized with NMR, elemental analysis (EA), size exclusion chromatography (SEC), and AFM. These methods established that PSI coatings deposited on surfaces were usually 2-5 nm thick and very hydrophobic. This prevents non-specific binding of proteins from the surface physically and chemically.

Proteins were immobilized on inorganic surfaces in three ways: PSI coatings with biotin, protein G, or direct binding to IgG on immunological assays. Antibody immobilization and antigen detection were measured using AFM, ellipsometry (ELS), fluorescence microscopy, and interferometry. The nonspecific binding of sample proteins from the blocking coatings was confirmed to be very low through SEC development.

Indexing (details)


Subject
Analytical chemistry;
Polymers
Classification
0486: Analytical chemistry
0495: Polymers
Identifier / keyword
Pure sciences; Biocompatible; Coatings; Polysuccinimide
Title
A new biocompatible coating for bioanalytical devices based on PSI (polysuccinimide)
Author
Cho, Wonryeon
Number of pages
168
Publication year
2006
Degree date
2006
School code
0183
Source
DAI-B 68/02, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
Advisor
Regnier, Fred E.
University/institution
Purdue University
University location
United States -- Indiana
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3251599
ProQuest document ID
305270588
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/305270588/abstract
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