Abstract/Details

Synthesis of surface functionalized nanoparticles for biorecognition, and controlled interactions with proteins


2006 2006

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Abstract (summary)

Bionanotechnology provides an attractive and unique arena for chemists, biologists and physicists due to its interdisciplinary nature. This thesis work has focused on using synthetic chemical tools to functionalize nanoparticles for biological applications. To this end, a ligand system featuring poly(ethylene glycol) (PEG) segments and chain-end functionalities was developed. These ligands were utilized to functionalized nanoparticles with different cores, including metallic (Au), semiconductor (CdSe and CdSe/ZnS), and magnetic (FePt and iron oxide) materials. Using these surface tailored nanoparticle scaffolds, surface binding of nanoparticles with enzyme α-chymotrypsin (ChT) was systematically studied. The ChT-nanoparticle interaction was characterized as electrostatically driven and reversible. Importantly, control over ChT structure and function was demonstrated on three levels: no binding, binding and denaturation, and binding without denaturation, dictated by the nanoparticle surface monolayer composition. The monolayer of nanoparticles can be tailored to not only control protein (ChT)-nanoparticle interactions, but also impact enzyme-substrate interactions, which results in enhanced ChT chemoselectivity towards substrates with different charges. In addition, the PEGylated nanoparticles have been explored to stabilize enzyme to stresses found in real-world bio-catalysis. The combination of the unique attributes of the nanoparticle cores and the function of the monolayer periphery provides numerous opportunities in creation of multi-functional nano-materials that are useful in biological and material applications.

Indexing (details)


Subject
Organic chemistry
Classification
0490: Organic chemistry
Identifier / keyword
Pure sciences; Biorecognition; Nanoparticles; Proteins; Surface functionalization
Title
Synthesis of surface functionalized nanoparticles for biorecognition, and controlled interactions with proteins
Author
Hong, Rui
Number of pages
170
Publication year
2006
Degree date
2006
School code
0118
Source
DAI-B 67/04, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
0542654024, 9780542654022
Advisor
Rotello, Vincent M.; Emrick, Todd S.
University/institution
University of Massachusetts Amherst
University location
United States -- Massachusetts
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3215893
ProQuest document ID
305306452
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/305306452
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