Abstract/Details

The influence of WC1 isoforms on γδ T cell function


2006 2006

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Abstract (summary)

WC1 molecules are an extensively diversified family of integral type 1 scavenger receptor cysteine rich (SRCR) membrane glycoproteins expressed on the surface of arteriodactyls such as sheep, cattle, and swine. The focus of this dissertation is on establishing the relevance of different forms of WC1 expressed on the surface of bovine γδT cells. The results demonstrate that identification of WC1 variant expression on these lymphocytes can be used to determine cellular responses to characterized methods of activation in vitro. In this way, discrete forms of WC1 are shown to be critical antigenic determinants for functionally distinct populations as demonstrated via CD25 expression, proliferation, and production of the inflammatory cytokine IFN-γ. At present, WC1 is the only unique determinant found on γδT cells other than the TCR that can be used to distinguish such populations. Genomic analysis carried out by others suggested the potential for more than 50 forms of WC1. This work is the first to show numerous biochemically distinct forms of plasma membrane-bound WC1 using 2D transfer methods. Currently, the bovine genome is sequenced but unassembled. Once complete, the procedures employed here can be used in conjunction with the genomic data and mass spectrometry to identify amino acid sequences of immunoprecipitated WC1 separated via 2D methods.

Indexing (details)


Subject
Immunology;
Veterinary services
Classification
0982: Immunology
0778: Veterinary services
Identifier / keyword
Health and environmental sciences; Biological sciences; Glycoproteins; WC1; gammadelta T cells
Title
The influence of WC1 isoforms on γδ T cell function
Author
Rogers, Aric N.
Number of pages
162
Publication year
2006
Degree date
2006
School code
0118
Source
DAI-B 67/01, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
054252211X, 9780542522116
Advisor
Baldwin, Cynthia L.
University/institution
University of Massachusetts Amherst
University location
United States -- Massachusetts
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3206197
ProQuest document ID
305309821
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/305309821
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