Water and starch chain mobility in cassava starch as monitored by NMR: Effects of heat-moisture treatments, growth conditions and harvest time

2003 2003

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Abstract (summary)

This study focuses on applying Nuclear Magnetic Resonance (NMR) to differentiate heterogeneous domains of starch Mobility and distribution of water and starch chains in cassava starch from crops grown in drought and rainy reasons, harvested at 6 months and 12 months, were investigated by deuterium (2H) solid state NMR and proton (1H) spin-spin relaxation time (T 2) distributions. Changes in water and starch chain mobility with heating, freezing, and acid hydrolysis were also studied.

In a very low moisture range (<5% dry basis), all water present was immobile (rigid) in both drought and rainy cassava (A-type crystals), and potato starches (B-type crystal). Thus, the amount of bound water was irrespective of degrees of crystallinity and crystal types. The “Monolayer” value estimated from water sorption isotherms (7–9% dry basis) over-estimated the level of bound water in starch (when compared with NMR data). With increasing water content, T, decreased to a minimum at a moisture range of 14–17% (dry basis) marking its onset of molecular plasticization of the starch chains. Packed beds of starch granules (54% water, dry basis) were subjected to heating and freezing treatments. NMR T2 distributions of water and starch protons were characterized with a continuous model. Intragranular water (T2 ∼ 3 ms, disappeared below 0°C) and extragranular water (T2 ∼ 20 ms) were observed. An extra long T2 (∼100 ms) population (postulated as water associated with leached amylose) was developed upon gelatinization.

The swelling and melting of starch from the drought crop were shifted to a higher temperature than those from the rainy crop resulting from a possible closer packing of rigid amylopectin and/or amylose chains. Upon gelatinization, rigid amylopectin (T2 ∼ 10 μs) disappeared, while the mobile amylopectin (in the amorphous region of semicrystalline lamellae, T2 ∼ 1 ms) increased. An additional fraction with very long T 2 (∼50 ms) emerged at 65°C and above indicating an increase in mobile/soluble starch.

This study demonstrated a possible use of NMR to monitor changes in starch chains and related water mobility in intact starch granules, which is a unique feature not offered by other methods.

Indexing (details)

Food science
0359: Food science
Identifier / keyword
Biological sciences; Cassava starch; Chain mobility; Starch; Water mobility
Water and starch chain mobility in cassava starch as monitored by NMR: Effects of heat-moisture treatments, growth conditions and harvest time
Chatakanonda, Pathama
Number of pages
Publication year
Degree date
School code
DAI-B 64/01, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
0493996877, 9780493996875
Chinachoti, Pavinee
University of Massachusetts Amherst
University location
United States -- Massachusetts
Source type
Dissertations & Theses
Document type
Dissertation/thesis number
ProQuest document ID
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
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