Abstract/Details

Microinjection into skin using microneedles


2006 2006

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Abstract (summary)

Although transdermal drug delivery provides patient convenience and sustained delivery in contrast to painful hypodermic injections, skin's low permeability prevents passive delivery of most drugs. The development of microneedles that penetrate the skin barrier, but are small enough not to stimulate nerves, has the potential to deliver drugs across skin in a painless way. In previous studies, solid silicon microneedles have been shown to increase skin permeability for transdermal diffusion of a wide range of molecules. However, controlled injection by convective flow into skin using hollow microneedles has remained a challenge. To address this challenge, the goals of this study were (i) to provide experimental measurements coupled with numerical simulations to quantitatively describe fluid mechanics of flow within microneedles over a range of experimental conditions and needle geometries, (ii) to demonstrate and study the effects of diffusion-based delivery of insulin to diabetic rats in vivo using solid and hollow microneedles and (iii) to determine the effect of experimental parameters on microinfusion through hollow microneedles into skin to optimize drug delivery protocols and identify rate-limiting barriers to flow.

Experimentally, we quantified the relationship between pressure drop and flow rate through microneedles as a function of fluid viscosity and microneedle length, diameter, and cone half-angle. Microneedle tip diameter and taper angle were the primary controlling parameters for flow through conically tapered microneedles as shown by numerical simulations. Flow rates over a range of 1.4--56 μl/s were achieved through microneedles (in the absence of skin) with pressure drops in the range of 4.6--196.5 kPa.

This work also studied the use of solid microneedle arrays to insert into the skin of diabetic animals for transdermal delivery of insulin. Blood glucose levels dropped by as much as 80% in diabetic hairless rats in vivo. Larger drops in blood glucose level and larger plasma insulin concentrations were shown due to higher donor solution insulin concentration, shorter microneedles insertion time and fewer repeated insertions.

The final scope of this work was to determine the effect of microneedle geometry and infusion protocols on microinfusion flow rate into skin in vitro. Infusion flow rates ranged from 21 to 1130 μl/h was demonstrated using these microneedles. The presence of a bevel at the microneedle tip, larger retraction distance and insertion depth, larger infusion pressure and the presence of hyaluronidase led to larger infusion flow rates.

Indexing (details)


Subject
Chemical engineering;
Biomedical research
Classification
0542: Chemical engineering
0541: Biomedical research
Identifier / keyword
Applied sciences; Convective flow; Drug delivery; Microinjection; Microneedles; Skin
Title
Microinjection into skin using microneedles
Author
Martanto, Wijaya
Number of pages
185
Publication year
2006
Degree date
2006
School code
0078
Source
DAI-B 67/09, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9780542861734
Advisor
Prausnitz, Mark R.
University/institution
Georgia Institute of Technology
University location
United States -- Georgia
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3233556
ProQuest document ID
305333071
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/305333071
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