Abstract/Details

The role of new mutations in evolution and cloning: Genetic analysis to identify the role of new beneficial mutations in increasing viability and salt tolerance in <i>Drosophila melanogaster</i> and the influence of deleterious mutations on cloning efficiency


2006 2006

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Abstract (summary)

Mutation and selection are major driving forces of evolution. However, the role of new beneficial mutation is considered negligible in almost all evolutionary processes. In most cases it is difficult to separate the effect of pre-existing genetic variation at low frequencies from new mutations. In this study, experiments with Drosophila melanogaster were conducted to study the effect of new beneficial mutations on increases in viability and in adaptation to increased toxic levels of dietary salt. Completely homozygous stocks were used in the experiments to eliminate the effect of pre-existing genetic variation. In the viability experiment, a modification of Dobzhansky and Spassy assay (1947) was used to demonstrate that new beneficial mutations can quickly increase viability in Drosophila populations of different sizes. By use of inbred homozygous stock it was demonstrated that adaptation of Drosophila to increased salt tolerance due to new beneficial mutations can also occur quickly. We have also documented that the accumulation of deleterious mutations can reduce cloning efficiency in somatic cells. Overall, the results of these studies support the view that new mutations along with selection can have a major influence on cloning and the evolution of organisms.

Indexing (details)


Subject
Genetics
Classification
0369: Genetics
Identifier / keyword
Biological sciences; Cloning; Salt tolerance; Viability
Title
The role of new mutations in evolution and cloning: Genetic analysis to identify the role of new beneficial mutations in increasing viability and salt tolerance in <i>Drosophila melanogaster</i> and the influence of deleterious mutations on cloning efficiency
Author
Azad, Priti
Number of pages
105
Publication year
2006
Degree date
2006
School code
0018
Source
DAI-B 67/08, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9780542856501
Advisor
Woodruff, Ron C.
University/institution
Bowling Green State University
University location
United States -- Ohio
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3233079
ProQuest document ID
305344666
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/305344666
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