Abstract/Details

Multi-scale measurements of the mechanical and transport properties of native and engineered articular cartilage


2006 2006

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Abstract (summary)

Articular cartilage is a dense connective tissue that lines the bony surfaces of diarthrodial joints, providing low friction and wear during joint motion. Mechanical loading of cartilage has been shown to be efficacious and necessary for the maintenance of normal metabolic activities of chondrocytes. The primary objective of this thesis is to examine the roles of physical stimulation and/or nutrient transport as possible mechanisms leading to such cartilaginous growth. The effect of dynamic loading on nutrient transport in tissue engineering of replacement constructs is investigated. Moreover, the effect of loading on the in-situ deformation behavior of the chondrocyte microenvironment is also examined.

Interpreting the effects of physical loading on cells hinges on our understanding of its surrounding extracellular matrix, and the structure-function relationships that the tissue possesses. Therefore the mechanical properties of cartilage were investigated, examining the anisotropy, inhomogeneity, and tension-compression nonlinearity of bovine shoulder and human patellar tissues. These findings are interpreted in the context of contributions from proteoglycan molecules and organization of type II collagen.

Based on the findings described in this thesis, mechanical loading appears to dramatically modulate the physical signals in and around chondrocytes, resulting in strain amplification in the cellular microenvironment relative to the applied deformation. Additionally, dynamic loading was found to enhance the rate of transport of growth factor like solutes into tissue constructs. Consequently, dynamic loading of cartilage or engineered constructs results in an environment that is simultaneously physically and metabolically stimulating.

Indexing (details)


Subject
Biomedical research
Classification
0541: Biomedical research
Identifier / keyword
Applied sciences; Articular cartilage; Osmotic pressure; Solute transport; Tissue engineering
Title
Multi-scale measurements of the mechanical and transport properties of native and engineered articular cartilage
Author
Chahine, Nadeen O.
Number of pages
251
Publication year
2006
Degree date
2006
School code
0054
Source
DAI-B 67/04, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9780542636981, 0542636980
Advisor
Ateshian, Gerard A.; Hung, Clark T.
University/institution
Columbia University
University location
United States -- New York
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3213485
ProQuest document ID
305358821
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/305358821
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