Abstract/Details

A newly identified hantavirus: The development of immunologic diagnostic assays and phylogenetic analysis for detection and characterization


2005 2005

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Abstract (summary)

We developed an enzyme linked immunosorbent assay (ELISA) utilizing a 59 amino acid synthetic peptide as an antigen for the serodiagnosis of hantavirus. We also developed a one-step real-time detection-PCR (RTD-PCR) assay for hantavirus detection in rodents and insectivores captured at various time points and trapsites between September 2000 and May 2004 in the Great Smoky Mountains National Park.

Blood samples from 305 rodents and insectivores were tested for anti-hantavirus IgG antibodies with our synthetic peptide ELISA and immunofluorescent assay (IFA). The sensitivity and specificity of this ELISA were comparable to those of the IFA using virus infected cells. The only seropositive animals detected were deer mice (Peromyscus maniculatus) and white-footed mice (Peromyscus leucopus). The overall estimated seroprevalence in GSMNP was 8.9% (27/305).

A total of 345 rodents and insectivores were tested by RTD-PCR and the overall estimated viral prevalence was 5.2% (18/345). We detected virus in 16 deer mice and white-footed mice, one smoky shrew (Sorex cinerus ) and one Southern red-backed vole (Clethrionomys gapperi ). This is the first reported case of a New World hantavirus being detected in a shrew and the first evidence of a hantavirus detected in a Clethrionomys spp.

We performed phylogenetic analysis on partial G1 and G 2 segments of the M gene and partial N segment of the S gene. We have designated this hantavirus strain Newfound Gap virus (NGV). The incongruous homologies of the NGV S and M segments to other closely related hantaviruses suggest that genetic reassortment resulting in a hybrid virus may have occurred. NGV possesses unique characteristics and is closely related to pathogenic strains that have resulted in HCPS case fatalities in this region.

Indexing (details)


Subject
Public health;
Immunology;
Microbiology;
Studies
Classification
0573: Public health
0982: Immunology
0410: Microbiology
Identifier / keyword
Health and environmental sciences; Biological sciences; Diagnostic; Hantavirus; Immunologic; Newfound Gap virus
Title
A newly identified hantavirus: The development of immunologic diagnostic assays and phylogenetic analysis for detection and characterization
Author
Lewis, Shawn Lee
Number of pages
173
Publication year
2005
Degree date
2005
School code
0226
Source
DAI-B 67/02, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
054254363X, 9780542543630
Advisor
New, John C., Jr.
University/institution
The University of Tennessee
University location
United States -- Tennessee
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3205789
ProQuest document ID
305369740
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/305369740
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