Abstract/Details

Genomic implications of oxidative stress in Caco -2 cells


2005 2005

Other formats: Order a copy

Abstract (summary)

To study the effects of oxidative stress on the intestinal tract, Caco-2 cells were treated with a 75 micromolar hydrogen peroxide (H 2O2) solution for 30 minutes. After RNA isolation and purification, analysis of gene expression was obtained via microarrays. Gene expression levels were confirmed by RT-PCR. Results indicate no expression changes in the antioxidant enzymes, catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase, which could be explained by their high kcat /Km ratios indicative of high catalytic efficiency. Gene expression of the cytochrome P450 enzyme CYP2B6 was upregulated 32%, which could indicate an important role for it in prevention or exacerbation of oxidative damage. CYP2B6 is one of approximately 60 CYP450 enzymes in humans responsible for the biotransformation of xenobiotics and endogenous organic compounds. Results indicated a repression in genes involved in lipid and cell membrane synthesis, such as fatty acid desaturase 2. Insulin-like growth factor 2 and S-phase kinase-associated protein 2, two genes involved in cell cycle progression and cell growth were down-regulated as well. Results indicated a repression in the transcriptional genes, Sp1 transcription factor and MYC-associated zinc finger protein. Also, DEAD box polypeptide 21 and DEAH box polypeptide 9, genes involved in RNA processing, were down-regulated. Other genes involved in translation, angiogenesis, DNA repair, protein processing, glucose production, cell to cell adhesion, and cell signaling were repressed as well. Results indicate that H2O2 inhibits the growth and proliferation of Caco-2 cells. This inhibition of Caco-2 cell proliferation and the induction of CYP2B6 may be novel key regulatory factors involved in protecting or damaging the intestine from oxidative stress.

Indexing (details)


Subject
Cellular biology;
Nutrition
Classification
0379: Cellular biology
0570: Nutrition
Identifier / keyword
Health and environmental sciences, Biological sciences, Caco-2, Hydrogen peroxide, Oxidative stress
Title
Genomic implications of oxidative stress in Caco -2 cells
Author
Herring, Theresa
Number of pages
167
Publication year
2005
Degree date
2005
School code
0138
Source
DAI-B 66/08, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9780542287558, 0542287552
Advisor
Cuppett, Susan
University/institution
The University of Nebraska - Lincoln
University location
United States -- Nebraska
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3186858
ProQuest document ID
305454853
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/305454853
Access the complete full text

You can get the full text of this document if it is part of your institution's ProQuest subscription.

Try one of the following:

  • Connect to ProQuest through your library network and search for the document from there.
  • Request the document from your library.
  • Go to the ProQuest login page and enter a ProQuest or My Research username / password.