Abstract/Details

Impact of ebola mucin-like domain on anti-glycoprotein antibodies induced by ebola virus-like particles


2010 2010

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Abstract (summary)

Ebolavirus Glycoprotein (GP), responsible for mediating host-cell attachment and membrane fusion, contains a heavily glycosylated mucin-like domain believed to “shield" GP from neutralizing antibodies. To test whether the mucin domain inhibits the production and function of anti-GP antibodies, we vaccinated mice five times with Ebola virus-like particles (VLPs) that express one of four glycoproteins; wild-type ebolavirus GP (EBGP), ebolavirus GP without its mucin-like domain (ΔMucGP), vesicular stomatitis virus G, and ebolavirus GP with a Crimean-Congo Hemorrhagic Fever Virus mucin domain substituted for the ebolavirus mucin-like domain (CMsubGP). Antibody in sera harvested from EBGP-, ΔMucGP- and CMsubGP-VLP immunized mice demonstrated similar binding against EBGP and its mutants when surface-expressed on CHO-K1 cells. However, significantly higher serum antibody titers from EBGP-VLP immunized mice as compared to VLP-ΔMucGP mouse sera were detected by western blot analysis of EBGP or its mutants. Neutralization assays are being performed to further characterize VLP-immunized mouse sera.

Indexing (details)


Subject
Virology;
Immunology
Classification
0720: Virology
0982: Immunology
Identifier / keyword
Health and environmental sciences; Biological sciences; Antibody; Ebolavirus; Glycoprotein; Mucin; Virus-like particle
Title
Impact of ebola mucin-like domain on anti-glycoprotein antibodies induced by ebola virus-like particles
Author
Tantral, Lee
Number of pages
45
Publication year
2010
Degree date
2010
School code
1353
Source
MAI 49/01M, Masters Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9781124154688
Advisor
Basler, Christopher F.
Committee member
Ting, Adrian; Tortorella, Domenico
University/institution
Mount Sinai School of Medicine of New York University
Department
Microbiology
University location
United States -- New York
Degree
M.S.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
1479692
ProQuest document ID
749762727
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/749762727
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