Abstract/Details

Quantitative analysis of single particle tracking experiments: Applying ecological methods in cellular biology

Rajani, Vishaal.   University of Alberta (Canada) ProQuest Dissertations Publishing,  2010. MR62987.

Abstract (summary)

Single-particle tracking (SPT) is a method used to study the diffusion of various molecules within the cell. SPT involves tagging proteins with optical labels and observing their individual two-dimensional trajectories with a microscope. The analysis of this data provides important information about protein movement and mechanism, and is used to create multistate biological models. One of the challenges in SPT analysis is the variety of complex environments that contribute to heterogeneity within movement paths. In this thesis, we explore the limitations of current methods used to analyze molecular movement, and adapt analytical methods used in animal movement analysis, such as correlated random walks and first-passage time variance, to SPT data of leukocyte function-associated antigen-1 (LFA-1) integral membrane proteins. We discuss the consequences of these methods in understanding different types of heterogeneity in protein movement behaviour, and provide support to results from current experimental work.

Indexing (details)


Subject
Cellular biology
Classification
0379: Cellular biology
Identifier / keyword
Biological sciences
Title
Quantitative analysis of single particle tracking experiments: Applying ecological methods in cellular biology
Author
Rajani, Vishaal
Number of pages
79
Degree date
2010
School code
0351
Source
MAI 49/02M, Masters Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
978-0-494-62987-1
University/institution
University of Alberta (Canada)
University location
Canada -- Alberta, CA
Degree
M.Sc.
Source type
Dissertation or Thesis
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
MR62987
ProQuest document ID
761634249
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
https://www.proquest.com/docview/761634249