Abstract/Details

Using self assembling amphiphilic nanomaterials to selectively extract and concentrate peptides for analysis by mass spectrometry


2010 2010

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Abstract (summary)

Very selective and highly sensitive ways to detect peptides or proteins of interest remain important goals in proteomics applications. This dissertation focuses on the use of amphiphilic polymeric materials that self-assemble as reverse micelles in apolar solvents as part of a liquid-liquid extraction methodology to selectively extract and concentrate peptides from aqueous solutions. After extraction the polymer-peptide mixtures are amenable to direct analysis by mass spectrometry (MS) using matrix-assisted laser desorption/ionization (MALDI). The polymeric materials that self-assemble in apolar solvents have charged interiors that allow oppositely-charged peptides to readily migrate into the aggregate's core, while similarly-charged peptides do not. This charge complementarity can be controlled by varying aqueous phase pH such that peptides having a narrow range of a pI values can be selectively isolated. Insights into the extraction mechanism and how these materials can potentially open the way for multidimensional separations were also studied. Furthermore, amino acid-specific covalent labels are introduced inside the reverse micelles to selectively react with the extracted peptides having the amino acid of interest. This reaction leads to a mass signature that can be used to identify peptides with amino acids of interest. In addition, bulk ampholytic gels and small commercial surfactants were investigated as extraction materials. Overall, our results reveal that the amphiphilic polymeric reverse micelles are far superior for selectively and efficiently extracting peptides from complex mixtures.

Indexing (details)


Subject
Analytical chemistry;
Biochemistry;
Polymer chemistry
Classification
0486: Analytical chemistry
0487: Biochemistry
0495: Polymer chemistry
Identifier / keyword
Pure sciences, Amphiphilic nanomaterials, Liquid liquid extraction, Peptides, Polymers, Reverse micelles, Self assembling
Title
Using self assembling amphiphilic nanomaterials to selectively extract and concentrate peptides for analysis by mass spectrometry
Author
Gomez-Escudero, Andrea
Number of pages
171
Publication year
2010
Degree date
2010
School code
0118
Source
DAI-B 71/12, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9781124319919
Advisor
Vachet, Richard W.
Committee member
Dinsmore, Anthony; Kaltashov, Igor; Thayumanavan, Sankaran
University/institution
University of Massachusetts Amherst
Department
Chemistry
University location
United States -- Massachusetts
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3427532
ProQuest document ID
816503228
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/816503228
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