Occurrence of tylosin-resistant enterococci in swine manure and tile drainage systems under no-till management

2010 2010

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Abstract (summary)

The sub-therapeutically use of tylosin by the swine industry provides selective pressure for the development of antibiotic resistance in gastrointestinal bacteria. Land application of swine manure into drained agricultural fields might accelerate the transport and dissemination of these microorganisms through soils, into tile drainage lines, and ultimately into surface waters; however, little is known about the hydrologic link between sources of antibiotic resistant bacteria and their transport in tile drainage systems. The objective of this study was to investigate the occurrence and transport of tylosin-resistant enterococci from tile-drained agricultural fields receiving semi-annual swine-waste applications. A field study was conducted at a farm near Nashua, IA in April (spring) and November (fall) of 2009. Liquid swine slurry from an operation feeding tylosin at sub-therapeutic levels was injected into no-till field plots. Resistance to tylosin in manure, soil and tile water was investigated by a phenotype-based method and polymerase chain reaction (PCR) using previously published primers, ermA, ermB, ermC, ermF, ermT, ermX, mefA, msrA . All enterococci in manure samples were resistant to tylosin in the spring, whereas about 68% of total enterococci in fall were resistant to tylosin. Average concentrations of total and tylosin-resistant enterococci in soil samples over the two experiments were 8.8 × 106 cfu/g of soil and 8.9 × 106 cfu/g of soil, respectively. Total and tylosin-resistant concentrations in tile water were significantly different during both the spring and fall experiments (P < 0.05). In drainage water, total enterococci ranged from 1.3 × 101 cfu/100 mL (fall) to 5.0 × 103 cfu/100 mL (spring) while tylosin-resistant enterococci ranged from 1.3 × 101 cfu/100 mL (fall) to 1.2 × 103 cfu/100 mL (spring). The greatest concentration of tylosin-resistant enterococci in manure and lowest in drainage water suggest that enterococci lose resistance as selective pressure from antibiotic residues decrease. Five macrolide-resistance genes namely ermB, ermC, ermF, ermT, msrAwere detected in greater than 9% of the samples. On average, most isolates (97%) harbored msrA, while only 9.5% of total isolates contains ermT gene and 9% contain ermC gene. The ermF gene was found in 156 out of 200 isolates (78%) and ermB gene was detected in 138 isolates (69%). Only four strains representing 2.0% of the total strains contains all five genes. The study results confirmed the hydrologic connection between the tylosin resistant enterococci and macrolide resistant genes in swine manure and their presence in tile water when manure is applied to agricultural fields followed by rainfall event. This work suggests that application of liquid manure from swine facilities administering sub-therapeutic doses of tylosin to tile-drained lands will promote the transport of antibiotic resistant bacteria through soil and tile lines, thus contaminating nearby soils and waters.

Indexing (details)

Agricultural engineering;
Environmental science
0410: Microbiology
0539: Agricultural engineering
0768: Environmental science
Identifier / keyword
Health and environmental sciences; Applied sciences; Biological sciences; Antibiotics; Microbial transport; No-till; Swine waste; Tile drainage; Tylosin resistant enterococci
Occurrence of tylosin-resistant enterococci in swine manure and tile drainage systems under no-till management
Hoang, Trang Thi Thu
Number of pages
Publication year
Degree date
School code
MAI 49/03M, Masters Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
Soupir, Michelle L.
Committee member
Bhandari, Alok; Moorman, Thomas B.
Iowa State University
Agricultural and Biosystems Engineering
University location
United States -- Iowa
Source type
Dissertations & Theses
Document type
Dissertation/thesis number
ProQuest document ID
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
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