Abstract/Details

Studies on Nanoparticle Based Avian Influenza Vaccines to Present Immunogenic Epitopes of the Virus with Concentration on Ectodomain of Matrix 2 (M2e) Protein


2011 2011

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Abstract (summary)

Avian influenza is an infectious disease of avian species caused by type A influenza viruses with a significant economic impact on the poultry industry. Vaccination is the main prevention strategy in many countries worldwide. However, available vaccines elicit antibodies against two major surface protein of the virus hemagglutinin (HA) and neuraminidase (NA), where they constantly change by point mutations. Influenza viruses can also easily undergo gene reassortment. Therefore, to protect chickens against new strain of avian influenza virus, as well as control and prevent virus spread among farms, new vaccines needed to be designed which is a tedious, time consuming and expensive. Recently, conserved regions of the influenza genome have been evaluated as possible universal vaccines to eliminate constant vaccine updates based on circulating virus. In this study, peptide nanotechnology was used to generate vaccine nanoparticles that carry the highly conserved external domain of matrix 2 protein (M2e). These nanoparticles presented M2e in monomeric or tetrameric forms, designated as PSC-M2e-CH and BNSC-M2eN-CH, respectively. First, to demonstrate immunogenicity of these nanoparticles, we measured anti-M2e antibody in chickens, particularly when a high dose was applied. Prior to vaccination-challenge study, the challenge dose were determined by oculonasal inoculation of 106 EID50 or 107.7 EID50 of low pathogenicity AI virus HSN2 followed by measuring cloacal and tracheal virus shedding. A biphasic virus shedding pattern was observed with two peaks of virus shedding at days 4 and 8 for both tracheal and cloacal swabs. The chickens infected with 107.7 EID 50 had significant virus shedding as compared with 106 EID50.

Based on results of mentioned studies, a vaccination-challenge study was conducted by using 75μg of each vaccine construct per inoculation (with and without adjuvant) and higher dose of virus for challenge. BN5C-M2e-CH with adjuvant significantly reduced the tracheal virus shedding compared with the positive challenge control and offered significant protection by expediting clearance of the virus in infected chickens. Reduction in cloacal virus shedding was not significant because cloacal shedding is low by nature. These results demonstrate that nanoparticles are a promising platform for immunogenic epitope delivery and M2e is a promising vaccine candidate against low pathogenicity avian influenza (LPAI) viruses.

Indexing (details)


Subject
Nanotechnology;
Virology;
Immunology
Classification
0652: Nanotechnology
0720: Virology
0982: Immunology
Identifier / keyword
Health and environmental sciences; Applied sciences; Biological sciences; Avian influenza; Ectodomain; Matrix 2 protein; Nanoparticles; Vaccines; Viruses
Title
Studies on Nanoparticle Based Avian Influenza Vaccines to Present Immunogenic Epitopes of the Virus with Concentration on Ectodomain of Matrix 2 (M2e) Protein
Author
Babapoor Dighaleh, Sankhiros
Number of pages
141
Publication year
2011
Degree date
2011
School code
0056
Source
DAI-B 72/10, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9781124776149
Advisor
Khan, Mazhar I.
University/institution
University of Connecticut
University location
United States -- Connecticut
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3464350
ProQuest document ID
883362586
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/883362586
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