Abstract/Details

Ghrelin as a Potential Biomarker for Obesity in African-American Adolescents


2011 2011

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Abstract (summary)

Obesity has become a global epidemic. The literature confirms that obesity results from various environmental factors superimposed on a genetic background. There are current interventions in place; however, they have not proven to be as effective as speculated. Exploring molecular pathways involved in energy metabolism could lead to the identification of novel therapies and biomarkers for obesity. Ghrelin is a novel orexigenic hormone that plays pivotal roles in a number of biologically important processes, including food consumption and long-term energy balance. Ghrelin is a gastric hormone that is increased during states of negative energy balance and decreased in states of positive energy balance. Leptin, which antagonizes orexigenic peptides like ghrelin, is an adipocyte-specific hormone that suppresses appetite. The level of serum leptin correlates with body fat mass. Targeting this and other physiological markers could unveil the molecular mechanisms involved in the development of obesity. The purpose of this study was to determine the serum ghrelin concentration of obese African-American adolescents with a family history of diabetes in a parent or grandparent. This study also aimed to identify the possible relationship of ghrelin, leptin, and insulin during a glucose tolerance test on obese African-American adolescents.

The plasma ghrelin concentration was measured using commercially available radioimmunoassay kits to identify the concentration of both total and active ghrelin. Total ghrelin is predominantly found in circulation. This study focuses on identifying total ghrelin concentration in both obese and non-obese African-American adolescents. The average plasma ghrelin concentration was higher in non-obese African-American adolescents (223 ± 17.6 pM) and lower in obese African-American adolescents (181 ± 17 pM). When ghrelin concentration was compared to BMI, individuals with higher BMIs were found to have lower concentrations of ghrelin in a fasted state. During a glucose tolerance test, the plasma ghrelin concentration varied from patient to patient. However, there was an overall decrease in plasma ghrelin during the glucose tolerance test on obese African-American adolescents.

Total ghrelin concentration was then compared to leptin and insulin data collected during a previous study on vitamin-D deficiency and insulin resistance. When comparing the data, ghrelin exhibited a negative relationship with both insulin (r= -0.71) and leptin (r= -0.56) during a glucose tolerance test. Therefore, high levels of insulin are associated with lower levels of ghrelin and high levels of ghrelin are associated with lower levels of leptin. Interestingly, in comparing these three hormones, we found that both leptin and ghrelin exhibited little change during the glucose tolerance test. These findings suggest that obese adolescents in this study have developed a resistance to the action of both ghrelin and leptin. These findings add to the current understanding of various hormones involved in obesity and create potential avenues to explore in both obesity and diabetes research.

Indexing (details)


Subject
African American Studies;
Molecular biology;
Black studies;
Endocrinology;
Biochemistry
Classification
0296: African American Studies
0307: Molecular biology
0325: Black studies
0409: Endocrinology
0487: Biochemistry
Identifier / keyword
Social sciences; Pure sciences; Biological sciences; African-American adolescents; Ghrelin; Insulin; Leptin; Obesity; Type-2 diabetes
Title
Ghrelin as a Potential Biomarker for Obesity in African-American Adolescents
Author
Fluitt, Maurice Bruce
Number of pages
79
Publication year
2011
Degree date
2011
School code
0088
Source
MAI 50/01M, Masters Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9781124826684
Advisor
Sridhar, Rajagopala; Gambhir, Kanwal K.
Committee member
Gambhir, Kanwal K.; Headings, Verle; Sridhar, Rajagopala
University/institution
Howard University
Department
Genetics and Human Genetics
University location
United States -- District of Columbia
Degree
M.S.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
1497756
ProQuest document ID
888050329
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/888050329
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