Abstract/Details

Studies on the biosynthesis of prenylated indole secondary metabolites from <i>Aspergillus versicolor</i> and <i>Aspergillus sp.</i>; and A novel approach to tumor specific drug delivery: Use of a naphthyridine drug linker with a DNA hairpin


2011 2011

Other formats: Order a copy

Abstract (summary)

Herein are documented our efforts in two projects, beginning with studies toward elucidating the biosynthesis of prenylated indole alkaloids from two different Aspergillus species. Marine-derived Aspergillus sp. and terrestrial-derived Aspergillus versicolor were found to produce antipodal metabolites, in which we have developed several putative biosynthetic pathways to determine the enantio-diverging point of these fungal cultures. Through the synthesis of several potential intermediates, both with and without isotopic labeling, as well as through bioinformatics analysis of both the (−)- and (+)-notoamide biosynthetic gene clusters, significant progress has been made toward identifying a single biosynthetic precursor that serves as an intermediate to the postulated enantio-diverging event, the intramolecular hetero Diels-Alder cycloaddition.

In the second project discussed, through collaboration with Dr. James Berenson at the University of California, Los Angeles, we have developed a novel tumor specific drug delivery system. Two naphthyridine-drug derivatives were synthesized and conjugated to a modified DNA oligonucleotide specifically targeted for multiple myeloma cells. The oligonucleotide-drug conjugate was successfully delivered and activated specifically within RMI8226 multiple myeloma cells.

Indexing (details)


Subject
Biochemistry;
Organic chemistry;
Pharmacy sciences
Classification
0487: Biochemistry
0490: Organic chemistry
0572: Pharmacy sciences
Identifier / keyword
Health and environmental sciences; Pure sciences; Aspergillus versicolor; DNA hairpin; Naphthyridine; Prenylated indole secondary metabolites; Tumor specific drug delivery
Title
Studies on the biosynthesis of prenylated indole secondary metabolites from <i>Aspergillus versicolor</i> and <i>Aspergillus sp.</i>; and A novel approach to tumor specific drug delivery: Use of a naphthyridine drug linker with a DNA hairpin
Author
Finefield, Jennifer M.
Number of pages
409
Publication year
2011
Degree date
2011
School code
0053
Source
DAI-B 72/12, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9781124890388
Advisor
Williams, Robert M.
Committee member
Elliott, Mike; Kennan, Alan; Rovis, Tomislav; Thamm, Doug
University/institution
Colorado State University
Department
Chemistry
University location
United States -- Colorado
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3473974
ProQuest document ID
896616940
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/896616940
Access the complete full text

You can get the full text of this document if it is part of your institution's ProQuest subscription.

Try one of the following:

  • Connect to ProQuest through your library network and search for the document from there.
  • Request the document from your library.
  • Go to the ProQuest login page and enter a ProQuest or My Research username / password.