Abstract/Details

Mass spectrometric analysis of monolayer protected nanoparticles


2012 2012

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Abstract (summary)

Monolayer protected nanoparticles (NPs) include an inorganic core and a monolayer of organic ligands. The wide variety of core materials and the tunable surface monolayers make NPs promising materials for numerous applications. Concerns related to unforeseen human health and environmental impacts of NPs have also been raised. In this thesis, new analytical methods based on mass spectrometry are developed to understand the fate, transport, and biodistributions of NPs in the complex biological systems. A laser desorption/ionization mass spectrometry (LDI-MS) method has been developed to characterize the monolayers on NP surface. LDI-MS allows multiple NPs taken up by cells to be measured and quantified in a multiplexed fashion. The correlations between surface properties of NPs and cellular uptake have also been explored. LDI-MS is further coupled with inductively coupled plasma mass spectrometry (ICP-MS) to quantitatively measure monolayer stability of gold NPs (AuNPs) and quantum dots (QDs), respectively, in live cells. This label-free approach allows correlating monolayer structure and particle size with NP stability in various cellular environments. Finally, uptake, distribution, accumulation, and excretion of NPs in higher order organisms, such as fish and plants, have been investigated to understand the environmental impact of nanomaterials. The results indicate that surface chemistry is a primary determinant. NPs with hydrophilic surfaces are substantially less toxic and present a lower degree of bioaccumulation, making these nanomaterials attractive for sustainable nanotechnology.

Indexing (details)


Subject
Analytical chemistry;
Inorganic chemistry;
Organic chemistry;
Nanoscience;
Materials science
Classification
0486: Analytical chemistry
0488: Inorganic chemistry
0490: Organic chemistry
0565: Nanoscience
0794: Materials science
Identifier / keyword
Applied sciences; Pure sciences; Biodistributions; Drug delivery; Gold nanoparticles; Laser desorption ionization; Monolayer protected nanoparticles
Title
Mass spectrometric analysis of monolayer protected nanoparticles
Author
Zhu, Zhengjiang
Number of pages
156
Publication year
2012
Degree date
2012
School code
0118
Source
DAI-B 73/06, Dissertation Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9781267210128
Advisor
Rotello, Vincent M.; Vachet, Richard W.
Committee member
Chambers, James J.; Eyles, Stephen J.
University/institution
University of Massachusetts Amherst
Department
Chemistry
University location
United States -- Massachusetts
Degree
Ph.D.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
3498415
ProQuest document ID
926571711
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/926571711
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