Abstract/Details

Identification of micro-RNAs targeting genes of PI3K/AKT pathway in ovarian cancer


2011 2011

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Abstract (summary)

Ovarian cancer is the leading cause of cancer-related death for females due to lack of specific early detection method. It is of great interest to find molecular-based biomarkers which are sensitive and specific to ovarian cancer for early diagnosis, prognosis and therapeutics. miRNAs have been proposed to be potential biomarkers that could be used in cancer prevention and therapeutics. The current study analyzed the miRNA and mRNA expression data extracted from the Cancer Genome Atlas (TCGA) database. Using simple linear regression and multiple regression models, we found 71 miRNA-mRNA pairs which were negatively associated between 56 miRNAs and 24 genes of PI3K/AKT pathway. Among these miRNA and mRNA target pairs, 9 of them were in agreement with the predictions from the most commonly used target prediction programs including miRGen, miRDB, miRTarbase and miR2Disease. These shared miRNA-mRNA pairs were considered to be the most potential genes that were involved in ovarian cancer. Furthermore, 4 of the 9 target genes encode cell cycle or apoptosis related proteins including Cyclin D1, p21, FOXO1 and Bcl2, suggesting that their regulator miRNAs including miR-16, miR-96 and miR-21 most likely played important roles in promoting tumor growth through dysregulated cell cycle or apoptosis. miR-96 was also found to directly target IRS-1. In addition, the results showed that miR-17 and miR-9 may be involved in ovarian cancer through targeting JAK1. This study might provide evidence for using miRNA or miRNA profile as biomarker.

Indexing (details)


Subject
Molecular biology;
Biostatistics;
Genetics;
Oncology
Classification
0307: Molecular biology
0308: Biostatistics
0369: Genetics
0992: Oncology
Identifier / keyword
Health and environmental sciences, Biological sciences, Mirna, Mrna, Ovarian cancer, Pi3k/akt pathway, Potential biomarkers
Title
Identification of micro-RNAs targeting genes of PI3K/AKT pathway in ovarian cancer
Author
Wang, Yuling
Number of pages
50
Publication year
2011
Degree date
2011
School code
0219
Source
MAI 50/05M, Masters Abstracts International
Place of publication
Ann Arbor
Country of publication
United States
ISBN
9781267240002
Advisor
Xiong, Momiao
Committee member
Baraniuk, Sarah; Kapadia, Asha S.
University/institution
The University of Texas School of Public Health
Department
Biostatistics
University location
United States -- Texas
Degree
M.S.
Source type
Dissertations & Theses
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
1507200
ProQuest document ID
940885985
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
http://search.proquest.com/docview/940885985
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