Abstract

There a few reports of rhodamine-based fluorescent sensors for selective detection of only Al3+, due to the challenge of identifying a suitable ligand for binding Al3+ ion. The use of fluorophore moieties appended to a polymer backbone for sensing applications is far from mature. Here, we report a new fluorescent probe/monomer 4 and its ROMP derived polymer P for specific detection of Al3+ ions. Both monomer 4 and its polymer P exhibit high selectivity toward only Al3+ with no interference from other metal ions, having a limit detection of 0.5 and 2.1 µM, respectively. The reversible recognition of monomer 4 and P for Al3+ was also proved in presence of Na2EDTA by both UV–Vis and fluorometric titration. The experimental data indicates the behavior of 4 and P toward Al3+ is pH independent in medium conditions. In addition, the switch-on luminescence response of 4 at acidic pH (0 < 5.0), allowed us to specifically stain lysosomes (pH ~ 4.5–5.0) in live cells.

Details

Title
Multifunctional rhodamine B appended ROMP derived fluorescent probe detects Al3+ and selectively labels lysosomes in live cells
Author
Reddy, Gandra Upendar 1 ; Courjaret Raphael 2 ; Machaca Khaled 2 ; Al-Hashimi, Mohammed 1 ; Bazzi, Hassan S 3 

 Texas A&M University at Qatar, Department of Chemistry, Doha, Qatar (GRID:grid.412392.f) 
 Weill Cornell Medicine Qatar, Department of Physiology and Biophysics, Doha, Qatar (GRID:grid.412392.f) 
 Texas A&M University at Qatar, Department of Chemistry, Doha, Qatar (GRID:grid.412392.f); Texas A&M University, Department of Materials Science & Engineering, College Station, USA (GRID:grid.264756.4) (ISNI:0000 0004 4687 2082) 
Publication year
2020
Publication date
2020
Publisher
Nature Publishing Group
e-ISSN
20452322
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2471558711
Copyright
© The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.