Abstract/Details

Genetic variation in native European populations of Pinus sylvestris (L.) (BL)

Soranzo, N.   University of Dundee (United Kingdom) ProQuest Dissertations & Theses,  1999. U531406.

Abstract (summary)

This thesis represents a study of genetic variation within native populations of the natural range of Pinus sylvestris (L.) in Europe. Genetic analysis was performed using markers specific for the nuclear, chloroplast and mitochondrial genomes. In most conifers, these genomes are inherited along distinct parental lineages (biparental, paternal and maternal respectively), therefore comparative analysis of specific markers provides information on the profiles of gene dispersal via pollen and seed. PCR-based assays were developed and DNA amplified from 23 Scots pine populations located within three distinct geographical regions in Europe, namely Scotland, the Iberian Peninsula and eastern Europe. The study of the nuclear variation was conducted using microsatellite markers, and restricted to the Iberian stands only. High levels of within-population genetic diversity were detected with nuclear SSRs throughout Spain (average Hc= 0.622), with low levels of population differentiation (3.5%; p<IMG WIDTH=10 HEIGHT=24 ALIGN=MIDDLE SRC="/maths/leq.gif"> 0.01). Analysis of chloroplast microsatellite loci detected analogously high levels of genetic variation throughout Europe (average H = 0.967), and low differentiation among populations (2%; p <IMG WIDTH=10 HEIGHT=24 ALIGN=MIDDLE SRC="/maths/leq.gif">0.05) and among the three geographic regions (1%; p<IMG WIDTH=10 HEIGHT=24 ALIGN=MIDDLE SRC="/maths/leq.gif">0.05).

The application of sequence-tagged site markers, based on the amplification of the trnT-trnL chloroplastic region, indicated a similarly low degree of genetic structuring. The development of polymorphic PCR-based markers for the mitochondrial genome proved to be particularly difficult due to the low rates of nucleotide substitution. During the course of this project, approximately 10 kilobases of mitochondrial DNA was sequenced and characterised. A polymorphic mitochondrial marker was developed based on the amplification of the nad1 intron. The distribution of haplotypes yielded significant population differentiation within Spain (59%; p <IMG WIDTH=10 HEIGHT=24 ALIGN=MIDDLE SRC="/maths/leq.gif">0.001) and between the Iberian and the remaining populations (84%; p <IMG WIDTH=10 HEIGHT=24 ALIGN=MIDDLE SRC="/maths/leq.gif">0.001), and identified a marked geographical structure typical of seed-specific markers.

Indexing (details)


Subject
Genetics
Classification
0369: Genetics
Identifier / keyword
(UMI)AAIU531406; Biological sciences
Title
Genetic variation in native European populations of Pinus sylvestris (L.) (BL)
Author
Soranzo, N.
Number of pages
1
Degree date
1999
School code
0277
Source
DAI-C 71/11, Dissertation Abstracts International
University/institution
University of Dundee (United Kingdom)
University location
Scotland
Degree
Ph.D.
Source type
Dissertation or Thesis
Language
English
Document type
Dissertation/Thesis
Dissertation/thesis number
U531406
ProQuest document ID
900241397
Copyright
Database copyright ProQuest LLC; ProQuest does not claim copyright in the individual underlying works.
Document URL
https://www.proquest.com/docview/900241397